Applications of radio frequency cold plasma treatment of polypropylene PCR plates

D.N. Howbrook; Dipak Sarker; Andrew Lloyd; A. Louwrier

doi:10.1023/A:1021335924788

Applications of radio frequency cold plasma treatment of polypropylene PCR plates

D.N. Howbrook, Dipak Sarker, Andrew Lloyd, A. Louwrier

Research output: Contribution to journal › Article › peer-review

Abstract

Radio frequency plasma treatment of 96-well PCR polypropylene plate, using O2, decreased DNA adsorption relative to untreated plates. Furthermore, radio frequency plasma treatment with a N2/H2 gas mixture increased DNA adsorption. This technique provides a simple means of modifying DNA adsorption to PCR polypropylene plates for various applications.

Original language	English
Pages (from-to)	2071-2074
Number of pages	4
Journal	Biotechnology Letters
Volume	24
Issue number	24
DOIs	https://doi.org/10.1023/A:1021335924788
Publication status	Published - Dec 2002
Event	IOP Plasma Physics Conference - Loughborough, United Kingdom Duration: 23 Apr 2019 → 26 Apr 2019 Conference number: 46 http://plasma2019.iopconfs.org/home

Keywords

DNA
PCR
polypropylene
radiofrequency plasma
surface modification

Access to Document

10.1023/A:1021335924788Licence: Unspecified

http://link.springer.com/article/10.1023%2FA%3A1021335924788Licence: Unspecified

Dimension Atomic Force Microscope (AFM)
Facility/equipment: Equipment
Environmental Scanning Electron Microscope EVO SEM
Facility/equipment: Equipment
Field Emission Scanning Electron Microscope FEG-SEM
Facility/equipment: Equipment

1 Research degree

Masters Degree by Research (France) - Mr Victor Jasny (University of Tours at Blois)
Sarker, D. (Supervisor)
1 Mar 2020 → 1 Aug 2020
Activity: External examination and supervision › Research degree

Cite this

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title = "Applications of radio frequency cold plasma treatment of polypropylene PCR plates",

abstract = "Radio frequency plasma treatment of 96-well PCR polypropylene plate, using O2, decreased DNA adsorption relative to untreated plates. Furthermore, radio frequency plasma treatment with a N2/H2 gas mixture increased DNA adsorption. This technique provides a simple means of modifying DNA adsorption to PCR polypropylene plates for various applications.",

keywords = "DNA, PCR, polypropylene, radiofrequency plasma, surface modification",

author = "D.N. Howbrook and Dipak Sarker and Andrew Lloyd and A. Louwrier",

year = "2002",

month = dec,

doi = "10.1023/A:1021335924788",

language = "English",

volume = "24",

pages = "2071--2074",

journal = "Biotechnology Letters",

issn = "1573-6776",

number = "24",

note = "IOP Plasma Physics Conference ; Conference date: 23-04-2019 Through 26-04-2019",

url = "http://plasma2019.iopconfs.org/home",

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TY - JOUR

T1 - Applications of radio frequency cold plasma treatment of polypropylene PCR plates

AU - Howbrook, D.N.

AU - Sarker, Dipak

AU - Lloyd, Andrew

AU - Louwrier, A.

N1 - Conference code: 46

PY - 2002/12

Y1 - 2002/12

N2 - Radio frequency plasma treatment of 96-well PCR polypropylene plate, using O2, decreased DNA adsorption relative to untreated plates. Furthermore, radio frequency plasma treatment with a N2/H2 gas mixture increased DNA adsorption. This technique provides a simple means of modifying DNA adsorption to PCR polypropylene plates for various applications.

AB - Radio frequency plasma treatment of 96-well PCR polypropylene plate, using O2, decreased DNA adsorption relative to untreated plates. Furthermore, radio frequency plasma treatment with a N2/H2 gas mixture increased DNA adsorption. This technique provides a simple means of modifying DNA adsorption to PCR polypropylene plates for various applications.

KW - DNA

KW - PCR

KW - polypropylene

KW - radiofrequency plasma

KW - surface modification

U2 - 10.1023/A:1021335924788

DO - 10.1023/A:1021335924788

M3 - Article

SN - 1573-6776

VL - 24

SP - 2071

EP - 2074

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 24

T2 - IOP Plasma Physics Conference

Y2 - 23 April 2019 through 26 April 2019

ER -

Applications of radio frequency cold plasma treatment of polypropylene PCR plates

Abstract

Keywords

Access to Document

Fingerprint

Equipment

Dimension Atomic Force Microscope (AFM)

Environmental Scanning Electron Microscope EVO SEM

Field Emission Scanning Electron Microscope FEG-SEM

Activities

Masters Degree by Research (France) - Mr Victor Jasny (University of Tours at Blois)

Cite this