Abstract
Accelerated age-related-hearing-loss disrupts high-frequency hearing in inbred CD-1 mice. The p.Ala88Val (A88V) mutation in the gene coding for the gap-junction protein connexin30 (Cx30) protects the cochlear basal turn of adult CD-1Cx30A88V/A88V mice from degeneration and rescues hearing. Here we report the passive compliance of the cochlear partition and active frequency tuning of the basilar membrane are enhanced in the cochleae of CD-1Cx30A88V/A88V compared to CBA/J mice with sensitive high-frequency hearing, suggesting gap-junctions contribute to passive cochlear mechanics and energy distribution in the active cochlea. Surprisingly, the endocochlear potential that drives mechanoelectrical transduction currents in outer hair cells (OHCs) and hence cochlear amplification is greatly reduced in CD-1Cx30A88V/A88V mice. Yet, the saturating amplitudes of cochlear microphonic potentials in CD-1Cx30A88V/A88V and CBA/J mice are comparable. Although not conclusive, these results are compatible with the proposal that OHC transmembrane potentials, determined mainly by potentials extracellular to the OHCs, drive somatic electromotility.
| Original language | English |
|---|---|
| Title of host publication | To the Ear and Back Again - Advances in Auditory Biophysics |
| Subtitle of host publication | Proceedings of the 13th Mechanics of Hearing Workshop |
| Publisher | American Institute of Physics Inc. |
| Volume | 1965 |
| ISBN (Electronic) | 9780735416703 |
| DOIs | |
| Publication status | Published - 31 May 2018 |
| Event | 13th Mechanics of Hearing Workshop: To the Ear and Back Again - Advances in Auditory Biophysics, MoH 2017 - St. Catharines, Canada Duration: 19 Jun 2017 → 24 Jun 2017 |
Publication series
| Name | AIP Conference Proceedings |
|---|---|
| Number | 1 |
| Volume | 1965 |
| ISSN (Print) | 0094-243X |
| ISSN (Electronic) | 1551-7616 |
Workshop
| Workshop | 13th Mechanics of Hearing Workshop: To the Ear and Back Again - Advances in Auditory Biophysics, MoH 2017 |
|---|---|
| Country | Canada |
| City | St. Catharines |
| Period | 19/06/17 → 24/06/17 |
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Bibliographical note
Roles for gap-junctions in cochlear amplification and micromechanics exposed by a conexin 30 mutation, Victoria A. Lukashkina, Snezana Levic, Nicola Strenzke, Andrei N. Lukashkin and Ian J. Russell, AIP Conference Proceedings 2018 1965:1Cite this
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Roles for gap-junctions in cochlear amplification and micromechanics exposed by a conexin 30 mutation. / Lukashkina, Victoria A.; Levic, Snezana; Strenzke, Nicola; Lukashkin, Andrei N.; Russell, Ian J.
To the Ear and Back Again - Advances in Auditory Biophysics: Proceedings of the 13th Mechanics of Hearing Workshop. Vol. 1965 American Institute of Physics Inc., 2018. 100001 (AIP Conference Proceedings; Vol. 1965, No. 1).Research output: Chapter in Book/Conference proceeding with ISSN or ISBN › Conference contribution with ISSN or ISBN
TY - GEN
T1 - Roles for gap-junctions in cochlear amplification and micromechanics exposed by a conexin 30 mutation
AU - Lukashkina, Victoria A.
AU - Levic, Snezana
AU - Strenzke, Nicola
AU - Lukashkin, Andrei N.
AU - Russell, Ian J.
N1 - Roles for gap-junctions in cochlear amplification and micromechanics exposed by a conexin 30 mutation, Victoria A. Lukashkina, Snezana Levic, Nicola Strenzke, Andrei N. Lukashkin and Ian J. Russell, AIP Conference Proceedings 2018 1965:1
PY - 2018/5/31
Y1 - 2018/5/31
N2 - Accelerated age-related-hearing-loss disrupts high-frequency hearing in inbred CD-1 mice. The p.Ala88Val (A88V) mutation in the gene coding for the gap-junction protein connexin30 (Cx30) protects the cochlear basal turn of adult CD-1Cx30A88V/A88V mice from degeneration and rescues hearing. Here we report the passive compliance of the cochlear partition and active frequency tuning of the basilar membrane are enhanced in the cochleae of CD-1Cx30A88V/A88V compared to CBA/J mice with sensitive high-frequency hearing, suggesting gap-junctions contribute to passive cochlear mechanics and energy distribution in the active cochlea. Surprisingly, the endocochlear potential that drives mechanoelectrical transduction currents in outer hair cells (OHCs) and hence cochlear amplification is greatly reduced in CD-1Cx30A88V/A88V mice. Yet, the saturating amplitudes of cochlear microphonic potentials in CD-1Cx30A88V/A88V and CBA/J mice are comparable. Although not conclusive, these results are compatible with the proposal that OHC transmembrane potentials, determined mainly by potentials extracellular to the OHCs, drive somatic electromotility.
AB - Accelerated age-related-hearing-loss disrupts high-frequency hearing in inbred CD-1 mice. The p.Ala88Val (A88V) mutation in the gene coding for the gap-junction protein connexin30 (Cx30) protects the cochlear basal turn of adult CD-1Cx30A88V/A88V mice from degeneration and rescues hearing. Here we report the passive compliance of the cochlear partition and active frequency tuning of the basilar membrane are enhanced in the cochleae of CD-1Cx30A88V/A88V compared to CBA/J mice with sensitive high-frequency hearing, suggesting gap-junctions contribute to passive cochlear mechanics and energy distribution in the active cochlea. Surprisingly, the endocochlear potential that drives mechanoelectrical transduction currents in outer hair cells (OHCs) and hence cochlear amplification is greatly reduced in CD-1Cx30A88V/A88V mice. Yet, the saturating amplitudes of cochlear microphonic potentials in CD-1Cx30A88V/A88V and CBA/J mice are comparable. Although not conclusive, these results are compatible with the proposal that OHC transmembrane potentials, determined mainly by potentials extracellular to the OHCs, drive somatic electromotility.
UR - http://www.scopus.com/inward/record.url?scp=85048211584&partnerID=8YFLogxK
U2 - 10.1063/1.5038499
DO - 10.1063/1.5038499
M3 - Conference contribution with ISSN or ISBN
AN - SCOPUS:85048211584
VL - 1965
T3 - AIP Conference Proceedings
BT - To the Ear and Back Again - Advances in Auditory Biophysics
PB - American Institute of Physics Inc.
ER -