Abstract
Rapid and efficient magnetization of human bone marrow stromal cells (BMSC) through functionalized magnetic nanoparticles (MNP). Methods: MNP were functionalized with poly(epsilon-lysine) dendrons exposing carboxybetaine residue (CB-MNP) to enhance binding to the cellular glycocalix. BMSC were incubated with CB-MNP or non-functionalized PAA-MNP for 5–30 min in suspension. Results: CB-MNP functionalization increased the magnetization efficiency by threefold. Remarkably, 66% of cells were magnetized after only 5 min and the maximum efficiency of >80% was reached by 15 min. BMSC viability, proliferation and differentiation were not impaired: actually, adipogenic and osteogenic differentiation were even improved. Conclusion: Carboxybetaine-dendron functionalization ensured rapid and efficient BMSC magnetization and allowed innovative suspension labeling, with a potential for bypassing adhesion culture of progenitors for regenerative medicine.
| Original language | English |
|---|---|
| Journal | Nanomedicine |
| DOIs | |
| Publication status | Published - 18 May 2016 |
Bibliographical note
This is an Accepted Manuscript of an article published Future Medicine in Nanomedicine on 18/05/2016, available online: http://www.futuremedicine.com/doi/10.2217/nnm-2016-0085Keywords
- adipogenesis
- carboxybetaine
- dendrimers
- magnetization
- nanoparticles
- osteogenesis
- suspension culture
- stem cells
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Matteo Santin
- School of Applied Sciences - Professor of Tissue Regeneration
- Centre for Precision Health and Translational Medicine
- Centre for Arts and Wellbeing
- Centre for Regenerative Medicine and Devices - Director
Person: Academic