Leukocyte Hsp72 mRNA transcription does not differ between males and females during heat acclimation

Jessica Mee, Oliver Gibson, J.A. Tuttle, L. Taylor, Peter Watt, Jonathan Doust, Neil Maxwell

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Purpose: Thermotolerance is an acquired state of increased cytoprotection achieved following single or repeated exposures to heat stress, in part characterised by changes in the intracellular 72kda heat shock protein (HSP72; HSPA1A). Females have demonstrated reduced exercise induced HSP72 in comparison to males. This study examined sex differences in heat shock protein 72 messenger ribonucleic acid (Hsp72 mRNA) transcription during heat acclimation (HA) to identify whether sex differences were a result of differential gene transcription. Methods: Ten participants (5M, 5F) performed ten, 90 min controlled hyperthermia [rectal temperature (Tre) ≥ 38.5°C] HA sessions over 12 d. Leukocyte Hsp72 mRNA was measured pre and post D1, D5, and D10, via Reverse transcription polymerase chain reaction (RT-QPCR). Results: HA was evidenced by a reduction in resting Tre (-0.4 ± 0.5°C) and resting heart rate [(HR); -13 ± 7 beats.min-1] following HA (p ≤ 0.05). During HA no difference (p > 0.05) was observed in ΔTre between males (D1 = 1.5 ± 0.2°C; D5 = 1.6 ± 0.4°C; D10 = 1.8 ± 0.3°C) and females (D1 = 1.5 ± 0.5°C; D5 = 1.4 ± 0.2°C; D10 = 1.8 ± 0.3°C). This was also true of mean Tre demonstrating equality of thermal stimuli for mRNA transcription and HA. There were no differences (p > 0.05) in Hsp72 mRNA expression between HA sessions or between males (D1 = +1.8 ± 1.5 fold; D5 = +2.0 ± 1.0 fold; D10 = +1.1 ± 0.4 fold) and females (D1 = +2.6 ± 1.8 fold; D5 = +1.8 ± 1.4 fold; D10 = +0.9 ± 1.9 fold). Conclusions: This experiment demonstrates that there is no difference in Hsp72 mRNA increases during HA between sexes when controlled hyperthermia HA is utilised. Gender specific differences in exercise-induced HSP72 reported elsewhere likely result from post-transcriptional events.
Original languageEnglish
Pages (from-to)549-556
Number of pages8
JournalTemperature
Volume3
Issue number4
DOIs
Publication statusPublished - 27 Jul 2016

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HSP72 Heat-Shock Proteins
Acclimatization
Leukocytes
Hot Temperature
RNA
Temperature
Sex Characteristics
Fever
Cytoprotection
Reverse Transcription

Bibliographical note

This is an Accepted Manuscript of an article published by Taylor & Francis in Temperature on 27/07/2016, available online: http://www.tandfonline.com/10.1080/23328940.2016.1214336.

Keywords

  • Controlled hyperthermia
  • males
  • females
  • thermotolerance
  • heat shock protein

Cite this

@article{6f783e5cd43d4936b800982366143fcf,
title = "Leukocyte Hsp72 mRNA transcription does not differ between males and females during heat acclimation",
abstract = "Purpose: Thermotolerance is an acquired state of increased cytoprotection achieved following single or repeated exposures to heat stress, in part characterised by changes in the intracellular 72kda heat shock protein (HSP72; HSPA1A). Females have demonstrated reduced exercise induced HSP72 in comparison to males. This study examined sex differences in heat shock protein 72 messenger ribonucleic acid (Hsp72 mRNA) transcription during heat acclimation (HA) to identify whether sex differences were a result of differential gene transcription. Methods: Ten participants (5M, 5F) performed ten, 90 min controlled hyperthermia [rectal temperature (Tre) ≥ 38.5°C] HA sessions over 12 d. Leukocyte Hsp72 mRNA was measured pre and post D1, D5, and D10, via Reverse transcription polymerase chain reaction (RT-QPCR). Results: HA was evidenced by a reduction in resting Tre (-0.4 ± 0.5°C) and resting heart rate [(HR); -13 ± 7 beats.min-1] following HA (p ≤ 0.05). During HA no difference (p > 0.05) was observed in ΔTre between males (D1 = 1.5 ± 0.2°C; D5 = 1.6 ± 0.4°C; D10 = 1.8 ± 0.3°C) and females (D1 = 1.5 ± 0.5°C; D5 = 1.4 ± 0.2°C; D10 = 1.8 ± 0.3°C). This was also true of mean Tre demonstrating equality of thermal stimuli for mRNA transcription and HA. There were no differences (p > 0.05) in Hsp72 mRNA expression between HA sessions or between males (D1 = +1.8 ± 1.5 fold; D5 = +2.0 ± 1.0 fold; D10 = +1.1 ± 0.4 fold) and females (D1 = +2.6 ± 1.8 fold; D5 = +1.8 ± 1.4 fold; D10 = +0.9 ± 1.9 fold). Conclusions: This experiment demonstrates that there is no difference in Hsp72 mRNA increases during HA between sexes when controlled hyperthermia HA is utilised. Gender specific differences in exercise-induced HSP72 reported elsewhere likely result from post-transcriptional events.",
keywords = "Controlled hyperthermia, males, females, thermotolerance, heat shock protein",
author = "Jessica Mee and Oliver Gibson and J.A. Tuttle and L. Taylor and Peter Watt and Jonathan Doust and Neil Maxwell",
note = "This is an Accepted Manuscript of an article published by Taylor & Francis in Temperature on 27/07/2016, available online: http://www.tandfonline.com/10.1080/23328940.2016.1214336.",
year = "2016",
month = "7",
day = "27",
doi = "10.1080/23328940.2016.1214336",
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}

Leukocyte Hsp72 mRNA transcription does not differ between males and females during heat acclimation. / Mee, Jessica; Gibson, Oliver; Tuttle, J.A.; Taylor, L.; Watt, Peter; Doust, Jonathan; Maxwell, Neil.

In: Temperature, Vol. 3, No. 4, 27.07.2016, p. 549-556.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Leukocyte Hsp72 mRNA transcription does not differ between males and females during heat acclimation

AU - Mee, Jessica

AU - Gibson, Oliver

AU - Tuttle, J.A.

AU - Taylor, L.

AU - Watt, Peter

AU - Doust, Jonathan

AU - Maxwell, Neil

N1 - This is an Accepted Manuscript of an article published by Taylor & Francis in Temperature on 27/07/2016, available online: http://www.tandfonline.com/10.1080/23328940.2016.1214336.

PY - 2016/7/27

Y1 - 2016/7/27

N2 - Purpose: Thermotolerance is an acquired state of increased cytoprotection achieved following single or repeated exposures to heat stress, in part characterised by changes in the intracellular 72kda heat shock protein (HSP72; HSPA1A). Females have demonstrated reduced exercise induced HSP72 in comparison to males. This study examined sex differences in heat shock protein 72 messenger ribonucleic acid (Hsp72 mRNA) transcription during heat acclimation (HA) to identify whether sex differences were a result of differential gene transcription. Methods: Ten participants (5M, 5F) performed ten, 90 min controlled hyperthermia [rectal temperature (Tre) ≥ 38.5°C] HA sessions over 12 d. Leukocyte Hsp72 mRNA was measured pre and post D1, D5, and D10, via Reverse transcription polymerase chain reaction (RT-QPCR). Results: HA was evidenced by a reduction in resting Tre (-0.4 ± 0.5°C) and resting heart rate [(HR); -13 ± 7 beats.min-1] following HA (p ≤ 0.05). During HA no difference (p > 0.05) was observed in ΔTre between males (D1 = 1.5 ± 0.2°C; D5 = 1.6 ± 0.4°C; D10 = 1.8 ± 0.3°C) and females (D1 = 1.5 ± 0.5°C; D5 = 1.4 ± 0.2°C; D10 = 1.8 ± 0.3°C). This was also true of mean Tre demonstrating equality of thermal stimuli for mRNA transcription and HA. There were no differences (p > 0.05) in Hsp72 mRNA expression between HA sessions or between males (D1 = +1.8 ± 1.5 fold; D5 = +2.0 ± 1.0 fold; D10 = +1.1 ± 0.4 fold) and females (D1 = +2.6 ± 1.8 fold; D5 = +1.8 ± 1.4 fold; D10 = +0.9 ± 1.9 fold). Conclusions: This experiment demonstrates that there is no difference in Hsp72 mRNA increases during HA between sexes when controlled hyperthermia HA is utilised. Gender specific differences in exercise-induced HSP72 reported elsewhere likely result from post-transcriptional events.

AB - Purpose: Thermotolerance is an acquired state of increased cytoprotection achieved following single or repeated exposures to heat stress, in part characterised by changes in the intracellular 72kda heat shock protein (HSP72; HSPA1A). Females have demonstrated reduced exercise induced HSP72 in comparison to males. This study examined sex differences in heat shock protein 72 messenger ribonucleic acid (Hsp72 mRNA) transcription during heat acclimation (HA) to identify whether sex differences were a result of differential gene transcription. Methods: Ten participants (5M, 5F) performed ten, 90 min controlled hyperthermia [rectal temperature (Tre) ≥ 38.5°C] HA sessions over 12 d. Leukocyte Hsp72 mRNA was measured pre and post D1, D5, and D10, via Reverse transcription polymerase chain reaction (RT-QPCR). Results: HA was evidenced by a reduction in resting Tre (-0.4 ± 0.5°C) and resting heart rate [(HR); -13 ± 7 beats.min-1] following HA (p ≤ 0.05). During HA no difference (p > 0.05) was observed in ΔTre between males (D1 = 1.5 ± 0.2°C; D5 = 1.6 ± 0.4°C; D10 = 1.8 ± 0.3°C) and females (D1 = 1.5 ± 0.5°C; D5 = 1.4 ± 0.2°C; D10 = 1.8 ± 0.3°C). This was also true of mean Tre demonstrating equality of thermal stimuli for mRNA transcription and HA. There were no differences (p > 0.05) in Hsp72 mRNA expression between HA sessions or between males (D1 = +1.8 ± 1.5 fold; D5 = +2.0 ± 1.0 fold; D10 = +1.1 ± 0.4 fold) and females (D1 = +2.6 ± 1.8 fold; D5 = +1.8 ± 1.4 fold; D10 = +0.9 ± 1.9 fold). Conclusions: This experiment demonstrates that there is no difference in Hsp72 mRNA increases during HA between sexes when controlled hyperthermia HA is utilised. Gender specific differences in exercise-induced HSP72 reported elsewhere likely result from post-transcriptional events.

KW - Controlled hyperthermia

KW - males

KW - females

KW - thermotolerance

KW - heat shock protein

U2 - 10.1080/23328940.2016.1214336

DO - 10.1080/23328940.2016.1214336

M3 - Article

VL - 3

SP - 549

EP - 556

JO - Temperature

JF - Temperature

SN - 2332-8959

IS - 4

ER -