In present work, Jatropha, Karanja (non-edible) and Cottonseed (edible) oils were mixed in the selected proportions and then converted to biomix methyl ester through transesterification process. Experiments were performed on a single cylinder non-road diesel engine with biomix methyl ester and results were compared with neat diesel, Jatropha, Karanja and Cottonseed biodiesel's. The major limitation observed in the biodiesel research is the fuel composition variability raised due to feedstock quality and production process parameters. Hence to have a sustainable biodiesel production based on available feedstocks of the region with balanced proportion of saturated and unsaturated fatty acids, use of biomix fuel is proposed on non-road diesel engine in this study. Non-road diesel engines are widely used in agriculture, power generation and construction equipment's which is also consuming lot of diesel and emitting more emissions. Use of renewable biomix fuel on non-road diesel engines will cut carbon foot print on the environment and it is better than biodiesel derived from a single feedstock based on the composition, fuel properties, performance and emissions. The results also indicated that biomix fuel has higher brake thermal efficiency, lower oxides of nitrogen and carbon-di-oxide emissions compared to biodiesel derived from single feedstock.