Development and evaluation of a two-step multiplex TaqMan real-time PCR assay for detection/quantification of different genospecies of Borrelia burgdorferi sensu lato

Keyphrases

• Multiplex Real-time PCR 100%
• Borrelia Burgdorferi Sensu Lato 100%
• Genospecies 100%
• Clinical Samples 75%
• Lusitania 75%
• Sensu Stricto 75%
• High Specificity 50%
• FlaB 50%
• Cerebrospinal Fluid Samples 50%
• PCR-targeting 50%
• Lyme Borreliosis 50%
• Genome Equivalent 50%
• Clinically Significant 25%
• Clinical Manifestations 25%
• Diagnostic Procedures 25%
• Early Diagnosis 25%
• Environmental Samples 25%
• Portugal 25%
• High Sensitivity 25%
• DNA Extracts 25%
• Quantitative PCR 25%
• Tropism 25%
• Laboratory Methods 25%
• Molecular Diagnostics 25%
• Actin Gene 25%
• Internal Control 25%
• Borrelia 25%
• Tetraplex Real-time PCR 25%
• Isolated Culture 25%
• Duplex PCR 25%
• 18S rDNA 25%
• PCR Protocol 25%
• Low Sensitivity 25%
• Specific Antibodies 25%

Immunology and Microbiology

• Real Time Polymerase Chain Reaction 100%
• Borreliella 100%
• Cerebrospinal Fluid 40%
• Actin 20%
• Window Period 20%
• Borrelia 20%
• Laboratory Technique 20%