Cross-platform transcriptomic profiling of the response to recombinant human erythropoietin

Guan Wang, Traci Kitaoka, Ali Crawford, Qian Mao, Andrew Hesketh, Fergus Guppy, Garrett I. Ash, Jason Liu, Mark Gerstein, Yannis Pitsiladis

Research output: Contribution to journalArticlepeer-review


RNA-seq has matured and become an important tool for studying RNA biology. Here we compared two RNA-seq (MGI DNBSEQ and Illumina NextSeq 500) and two microarray platforms (GeneChip Human Transcriptome Array 2.0 and Illumina Expression BeadChip) in healthy individuals administered recombinant human erythropoietin for transcriptome-wide quantification of differential gene expression. The results show that total RNA DNB-seq generated a multitude of target genes compared to other platforms. Pathway enrichment analyses revealed genes correlate to not only erythropoiesis and oxygen transport but also a wide range of other functions, such as tissue protection and immune regulation. This study provides a knowledge base of genes relevant to EPO biology through cross-platform comparisons and validation.
Original languageEnglish
Article number21705
JournalScientific Reports
Issue number1
Publication statusPublished - 4 Nov 2021

Bibliographical note

Funding Information:
We thank BGI Hong Kong for conducting the DNB-seq. This work was funded by the World Anti-Doping Agency (WADA) grant no. 08C19YP and ISF15E10YP to YPP. GIA was supported by a fellowship from the Office of Academic Affiliations at the United States Veterans Health Administration.


  • MGI DNB-seq
  • Illumina mRNA-seq
  • GeneChip Human Transcriptome Array 2.0
  • Illumina Expression BeadChip
  • Recombinant human erythropoietin
  • Differential gene expression
  • Biological pathways


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