RNA-seq has matured and become an important tool for studying RNA biology. Here we compared two RNA-seq (MGI DNBSEQ and Illumina NextSeq 500) and two microarray platforms (GeneChip Human Transcriptome Array 2.0 and Illumina Expression BeadChip) in healthy individuals administered recombinant human erythropoietin for transcriptome-wide quantification of differential gene expression. The results show that total RNA DNB-seq generated a multitude of target genes compared to other platforms. Pathway enrichment analyses revealed genes correlate to not only erythropoiesis and oxygen transport but also a wide range of other functions, such as tissue protection and immune regulation. This study provides a knowledge base of genes relevant to EPO biology through cross-platform comparisons and validation.
Bibliographical noteFunding Information:
We thank BGI Hong Kong for conducting the DNB-seq. This work was funded by the World Anti-Doping Agency (WADA) grant no. 08C19YP and ISF15E10YP to YPP. GIA was supported by a fellowship from the Office of Academic Affiliations at the United States Veterans Health Administration.
- MGI DNB-seq
- Illumina mRNA-seq
- GeneChip Human Transcriptome Array 2.0
- Illumina Expression BeadChip
- Recombinant human erythropoietin
- Differential gene expression
- Biological pathways