Preparation of hydrophobic and hydrophilic albumin microspheres and determination of surface carboxylic acid and amino residues

Angela Macadam, Z.B. Shafi, S.Elizabeth James, Claire Marriott, G.P. Martin

Research output: Contribution to journalArticle

Abstract

Albumin microspheres (MS) have been studied extensively as delivery systems for targeting drugs since they are biodegradable, non-toxic, relatively easy to prepare and their size range can be controlled. A method for albumin MS production was developed which was faster, processed larger quantities of starting material than previous methods, and had chemically reactive groupings on the MS surface to which ligands could be attached. Relatively hydrophobic, hydrophilic and also carboxymethylated MS were manufactured. The number of carboxylic acid residues was determined on the surface of these MS using 14C-glycine ethyl ester hydrochloride as a probe, and the number of amino groups was determined using 14C-sodium acetate as a probe. The number of carboxylic acid residues per unit surface area for the hydrophobic, hydrophilic and carboxymethylated MS was 2.1 × 104, 4.1 × 104 and 8.4 × 104, respectively, and the number of amino acid residues was 2.2 × 103, 5.0 × 102 and 5.0 × 102, respectively
Original languageEnglish
Pages (from-to)47-56
Number of pages10
JournalInternational Journal of Pharmaceutics
Volume151
Issue number1
DOIs
Publication statusPublished - 12 May 1997

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Carboxylic Acids
Microspheres
Albumins
Sodium Acetate
Ligands
Amino Acids

Keywords

  • albumin microspheres
  • hydrophobic
  • hydrophilic
  • carboxymethylated
  • crosslinking
  • glutaraldehyde
  • surface carboxylic acid residues
  • surface amino residues
  • C-14 glycine ethyl ester hydrochloride
  • C-14 sodium acetate

Cite this

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title = "Preparation of hydrophobic and hydrophilic albumin microspheres and determination of surface carboxylic acid and amino residues",
abstract = "Albumin microspheres (MS) have been studied extensively as delivery systems for targeting drugs since they are biodegradable, non-toxic, relatively easy to prepare and their size range can be controlled. A method for albumin MS production was developed which was faster, processed larger quantities of starting material than previous methods, and had chemically reactive groupings on the MS surface to which ligands could be attached. Relatively hydrophobic, hydrophilic and also carboxymethylated MS were manufactured. The number of carboxylic acid residues was determined on the surface of these MS using 14C-glycine ethyl ester hydrochloride as a probe, and the number of amino groups was determined using 14C-sodium acetate as a probe. The number of carboxylic acid residues per unit surface area for the hydrophobic, hydrophilic and carboxymethylated MS was 2.1 × 104, 4.1 × 104 and 8.4 × 104, respectively, and the number of amino acid residues was 2.2 × 103, 5.0 × 102 and 5.0 × 102, respectively",
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author = "Angela Macadam and Z.B. Shafi and S.Elizabeth James and Claire Marriott and G.P. Martin",
year = "1997",
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Preparation of hydrophobic and hydrophilic albumin microspheres and determination of surface carboxylic acid and amino residues. / Macadam, Angela; Shafi, Z.B.; James, S.Elizabeth; Marriott, Claire; Martin, G.P.

In: International Journal of Pharmaceutics, Vol. 151, No. 1, 12.05.1997, p. 47-56.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Preparation of hydrophobic and hydrophilic albumin microspheres and determination of surface carboxylic acid and amino residues

AU - Macadam, Angela

AU - Shafi, Z.B.

AU - James, S.Elizabeth

AU - Marriott, Claire

AU - Martin, G.P.

PY - 1997/5/12

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AB - Albumin microspheres (MS) have been studied extensively as delivery systems for targeting drugs since they are biodegradable, non-toxic, relatively easy to prepare and their size range can be controlled. A method for albumin MS production was developed which was faster, processed larger quantities of starting material than previous methods, and had chemically reactive groupings on the MS surface to which ligands could be attached. Relatively hydrophobic, hydrophilic and also carboxymethylated MS were manufactured. The number of carboxylic acid residues was determined on the surface of these MS using 14C-glycine ethyl ester hydrochloride as a probe, and the number of amino groups was determined using 14C-sodium acetate as a probe. The number of carboxylic acid residues per unit surface area for the hydrophobic, hydrophilic and carboxymethylated MS was 2.1 × 104, 4.1 × 104 and 8.4 × 104, respectively, and the number of amino acid residues was 2.2 × 103, 5.0 × 102 and 5.0 × 102, respectively

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