Preparation of hydrophobic and hydrophilic albumin microspheres and determination of surface carboxylic acid and amino residues

Angela Macadam, Z.B. Shafi, S.Elizabeth James, Claire Marriott, G.P. Martin

Research output: Contribution to journalArticle

Abstract

Albumin microspheres (MS) have been studied extensively as delivery systems for targeting drugs since they are biodegradable, non-toxic, relatively easy to prepare and their size range can be controlled. A method for albumin MS production was developed which was faster, processed larger quantities of starting material than previous methods, and had chemically reactive groupings on the MS surface to which ligands could be attached. Relatively hydrophobic, hydrophilic and also carboxymethylated MS were manufactured. The number of carboxylic acid residues was determined on the surface of these MS using 14C-glycine ethyl ester hydrochloride as a probe, and the number of amino groups was determined using 14C-sodium acetate as a probe. The number of carboxylic acid residues per unit surface area for the hydrophobic, hydrophilic and carboxymethylated MS was 2.1 × 104, 4.1 × 104 and 8.4 × 104, respectively, and the number of amino acid residues was 2.2 × 103, 5.0 × 102 and 5.0 × 102, respectively
Original languageEnglish
Pages (from-to)47-56
Number of pages10
JournalInternational Journal of Pharmaceutics
Volume151
Issue number1
DOIs
Publication statusPublished - 12 May 1997

Keywords

  • albumin microspheres
  • hydrophobic
  • hydrophilic
  • carboxymethylated
  • crosslinking
  • glutaraldehyde
  • surface carboxylic acid residues
  • surface amino residues
  • C-14 glycine ethyl ester hydrochloride
  • C-14 sodium acetate

Fingerprint Dive into the research topics of 'Preparation of hydrophobic and hydrophilic albumin microspheres and determination of surface carboxylic acid and amino residues'. Together they form a unique fingerprint.

  • Cite this