Plume image profiling of UV laser desorbed biomolecules

T. L. Merrigan; C. A. Hunniford; D. J. Timson; M. Catney; R. W. McCullough

doi:10.1063/1.3058978

Plume image profiling of UV laser desorbed biomolecules

T. L. Merrigan, C. A. Hunniford, D. J. Timson, M. Catney, R. W. McCullough

Research output: Contribution to journal › Article › peer-review

Abstract

An experimental system, based upon the techniques of UV and IR laser desorption with time of flight mass spectrometry, has been constructed to enable the production and characterization of neutral biomolecular targets. The feasibility of the laser desorption technique for the purpose of radiation interaction experiments is investigated here. Fluorescent dye tagging and laser induced fluorescence imaging has been used to help characterize the laser produced plumes of biomolecules revealing their spatial density profiles and temporal evolution. Peak target thicknesses of 2 × 10¹² molecules cm^-2 were obtained 30μs after laser desorption.

Original language	English
Pages (from-to)	176-181
Number of pages	6
Journal	AIP Conference Proceedings
Volume	1080
DOIs	https://doi.org/10.1063/1.3058978
Publication status	Published - 1 Dec 2008
Event	5th International Conference on Radiation Damage in Biomolecular Systems, RADAM 2008 - Debrecen, Hungary Duration: 13 Jun 2008 → 15 Jun 2008

Keywords

Biomolecules
Laser desorption
Mass spectrometry

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10.1063/1.3058978

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title = "Plume image profiling of UV laser desorbed biomolecules",

abstract = "An experimental system, based upon the techniques of UV and IR laser desorption with time of flight mass spectrometry, has been constructed to enable the production and characterization of neutral biomolecular targets. The feasibility of the laser desorption technique for the purpose of radiation interaction experiments is investigated here. Fluorescent dye tagging and laser induced fluorescence imaging has been used to help characterize the laser produced plumes of biomolecules revealing their spatial density profiles and temporal evolution. Peak target thicknesses of 2 × 1012 molecules cm-2 were obtained 30μs after laser desorption.",

keywords = "Biomolecules, Laser desorption, Mass spectrometry",

author = "Merrigan, {T. L.} and Hunniford, {C. A.} and Timson, {D. J.} and M. Catney and McCullough, {R. W.}",

year = "2008",

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doi = "10.1063/1.3058978",

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T1 - Plume image profiling of UV laser desorbed biomolecules

AU - Merrigan, T. L.

AU - Hunniford, C. A.

AU - Timson, D. J.

AU - Catney, M.

AU - McCullough, R. W.

PY - 2008/12/1

Y1 - 2008/12/1

N2 - An experimental system, based upon the techniques of UV and IR laser desorption with time of flight mass spectrometry, has been constructed to enable the production and characterization of neutral biomolecular targets. The feasibility of the laser desorption technique for the purpose of radiation interaction experiments is investigated here. Fluorescent dye tagging and laser induced fluorescence imaging has been used to help characterize the laser produced plumes of biomolecules revealing their spatial density profiles and temporal evolution. Peak target thicknesses of 2 × 1012 molecules cm-2 were obtained 30μs after laser desorption.

AB - An experimental system, based upon the techniques of UV and IR laser desorption with time of flight mass spectrometry, has been constructed to enable the production and characterization of neutral biomolecular targets. The feasibility of the laser desorption technique for the purpose of radiation interaction experiments is investigated here. Fluorescent dye tagging and laser induced fluorescence imaging has been used to help characterize the laser produced plumes of biomolecules revealing their spatial density profiles and temporal evolution. Peak target thicknesses of 2 × 1012 molecules cm-2 were obtained 30μs after laser desorption.

KW - Biomolecules

KW - Laser desorption

KW - Mass spectrometry

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M3 - Article

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SN - 0094-243X

VL - 1080

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EP - 181

JO - AIP Conference Proceedings

JF - AIP Conference Proceedings

T2 - 5th International Conference on Radiation Damage in Biomolecular Systems, RADAM 2008

Y2 - 13 June 2008 through 15 June 2008

ER -

Plume image profiling of UV laser desorbed biomolecules

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Keywords

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