Nucleotide sequence, heterologous expression and novel purification of DNA ligase from Bacillus stearothermophilus

James A. Brannigan; Stephen R. Ashford; Aidan J. Doherty; David J. Timson; Dale B. Wigley

doi:10.1016/S0167-4838(99)00122-3

Nucleotide sequence, heterologous expression and novel purification of DNA ligase from Bacillus stearothermophilus

James A. Brannigan, Stephen R. Ashford, Aidan J. Doherty, David J. Timson, Dale B. Wigley

Research output: Contribution to journal › Article › peer-review

Abstract

The gene for DNA ligase (EC 6.5.1.2) from thermophilic bacterium Bacillus stearothermophilus NCA1503 has been cloned and the complete nucleotide sequence determined. The ligase gene encodes a protein 670 amino acids in length. The gene was overexpressed in Escherichia coli and the enzyme has been purified to homogeneity. Preliminary characterisation confirms that it is a thermostable, NAD⁺-dependent DNA ligase. Copyright (C) 1999 Elsevier Science B.V.

Original language	English
Pages (from-to)	413-418
Number of pages	6
Journal	Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology
Volume	1432
Issue number	2
DOIs	https://doi.org/10.1016/S0167-4838(99)00122-3
Publication status	Published - 13 Jul 1999

Keywords

Bacillus stearothermophilus
DNA ligation
DNA replication
NAD
Thermostable protein

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10.1016/S0167-4838(99)00122-3

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title = "Nucleotide sequence, heterologous expression and novel purification of DNA ligase from Bacillus stearothermophilus",

abstract = "The gene for DNA ligase (EC 6.5.1.2) from thermophilic bacterium Bacillus stearothermophilus NCA1503 has been cloned and the complete nucleotide sequence determined. The ligase gene encodes a protein 670 amino acids in length. The gene was overexpressed in Escherichia coli and the enzyme has been purified to homogeneity. Preliminary characterisation confirms that it is a thermostable, NAD+-dependent DNA ligase. Copyright (C) 1999 Elsevier Science B.V.",

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Nucleotide sequence, heterologous expression and novel purification of DNA ligase from Bacillus stearothermophilus. / Brannigan, James A.; Ashford, Stephen R.; Doherty, Aidan J. et al.
In: Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology, Vol. 1432, No. 2, 13.07.1999, p. 413-418.

Research output: Contribution to journal › Article › peer-review

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T1 - Nucleotide sequence, heterologous expression and novel purification of DNA ligase from Bacillus stearothermophilus

AU - Brannigan, James A.

AU - Ashford, Stephen R.

AU - Doherty, Aidan J.

AU - Timson, David J.

AU - Wigley, Dale B.

PY - 1999/7/13

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KW - Bacillus stearothermophilus

KW - DNA ligation

KW - DNA replication

KW - NAD

KW - Thermostable protein

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ER -

Nucleotide sequence, heterologous expression and novel purification of DNA ligase from Bacillus stearothermophilus

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Keywords

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