Nano-analysis reveals high fraction of serotonin release during exocytosis from a gut epithelium model cell

Ying Wang; Chaoyi Gu; Bhavik Patel; Andrew Ewing

doi:10.1002/anie.202108193

Nano-analysis reveals high fraction of serotonin release during exocytosis from a gut epithelium model cell

Ying Wang, Chaoyi Gu, Bhavik Patel, Andrew Ewing

Research output: Contribution to journal › Article › peer-review

Abstract

Electrochemical methods were used to explore the exocytotic nature of serotonin (5-HT) release in human carcinoid BON cells, an in vitro human enterochromaffin cell model, to understand the mechanisms operating the release of gut-derived 5-HT in the intestinal mucosal epithelium. We show that the fractional vesicular 5-HT release in BON cells is 80% compared to previous work in pancreatic beta cells (34%). The fractional release increased from 80% in control BON cells to 87% with 5-HT preincubation and nearly 100% with the combination of 5-HT and the 5HT4 autoreceptor agonist, cisapride. Thus, partial release is the primary mechanism of exocytosis in BON cells, resulting in a variable amount of the vesicular content being released. Factors that control secretion of 5-HT from enterochromaffin cells or BON cells are important as partial release provides a mechanism for development of effective therapeutic strategies to treat gastrointestinal diseases.

Original language	English
Pages (from-to)	23552-23556
Number of pages	5
Journal	Angewandte Chemie-International Edition
Volume	60
Issue number	44
DOIs	https://doi.org/10.1002/anie.202108193
Publication status	Published - 7 Aug 2021

Bibliographical note

Funding Information:
We thank Dr. Mark R. Hellmich and Dr. Courtney M. Townsend at the University of Texas Medical Branch at Galveston for providing us the BON cells. We thank Dr. Xinwei Zhang for helpful discussion. The European Research Council (ERC Advanced Grant Project No 787534 NanoBioNext), Knut and Alice Wallenberg Foundation, and the Swedish Research Council (VR Grant No 2017-04366) are acknowledged for financial support.

Funding Information:
We thank Dr. Mark R. Hellmich and Dr. Courtney M. Townsend at the University of Texas Medical Branch at Galveston for providing us the BON cells. We thank Dr. Xinwei Zhang for helpful discussion. The European Research Council (ERC Advanced Grant Project No 787534 NanoBioNext), Knut and Alice Wallenberg Foundation, and the Swedish Research Council (VR Grant No 2017‐04366) are acknowledged for financial support.

Publisher Copyright:
© 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH

Keywords

BON cells
exocytosis
gut-derived 5-HT
nano-analysis
partial release
General Chemistry
Catalysis

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anie.202108193Final published version, 3.71 MBLicence: CC BY-NC-ND

Cite this

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title = "Nano-analysis reveals high fraction of serotonin release during exocytosis from a gut epithelium model cell",

abstract = "Electrochemical methods were used to explore the exocytotic nature of serotonin (5-HT) release in human carcinoid BON cells, an in vitro human enterochromaffin cell model, to understand the mechanisms operating the release of gut-derived 5-HT in the intestinal mucosal epithelium. We show that the fractional vesicular 5-HT release in BON cells is 80% compared to previous work in pancreatic beta cells (34%). The fractional release increased from 80% in control BON cells to 87% with 5-HT preincubation and nearly 100% with the combination of 5-HT and the 5HT4 autoreceptor agonist, cisapride. Thus, partial release is the primary mechanism of exocytosis in BON cells, resulting in a variable amount of the vesicular content being released. Factors that control secretion of 5-HT from enterochromaffin cells or BON cells are important as partial release provides a mechanism for development of effective therapeutic strategies to treat gastrointestinal diseases.",

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note = "Funding Information: We thank Dr. Mark R. Hellmich and Dr. Courtney M. Townsend at the University of Texas Medical Branch at Galveston for providing us the BON cells. We thank Dr. Xinwei Zhang for helpful discussion. The European Research Council (ERC Advanced Grant Project No 787534 NanoBioNext), Knut and Alice Wallenberg Foundation, and the Swedish Research Council (VR Grant No 2017-04366) are acknowledged for financial support. Funding Information: We thank Dr. Mark R. Hellmich and Dr. Courtney M. Townsend at the University of Texas Medical Branch at Galveston for providing us the BON cells. We thank Dr. Xinwei Zhang for helpful discussion. The European Research Council (ERC Advanced Grant Project No 787534 NanoBioNext), Knut and Alice Wallenberg Foundation, and the Swedish Research Council (VR Grant No 2017‐04366) are acknowledged for financial support. Publisher Copyright: {\textcopyright} 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH",

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AU - Ewing, Andrew

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N2 - Electrochemical methods were used to explore the exocytotic nature of serotonin (5-HT) release in human carcinoid BON cells, an in vitro human enterochromaffin cell model, to understand the mechanisms operating the release of gut-derived 5-HT in the intestinal mucosal epithelium. We show that the fractional vesicular 5-HT release in BON cells is 80% compared to previous work in pancreatic beta cells (34%). The fractional release increased from 80% in control BON cells to 87% with 5-HT preincubation and nearly 100% with the combination of 5-HT and the 5HT4 autoreceptor agonist, cisapride. Thus, partial release is the primary mechanism of exocytosis in BON cells, resulting in a variable amount of the vesicular content being released. Factors that control secretion of 5-HT from enterochromaffin cells or BON cells are important as partial release provides a mechanism for development of effective therapeutic strategies to treat gastrointestinal diseases.

AB - Electrochemical methods were used to explore the exocytotic nature of serotonin (5-HT) release in human carcinoid BON cells, an in vitro human enterochromaffin cell model, to understand the mechanisms operating the release of gut-derived 5-HT in the intestinal mucosal epithelium. We show that the fractional vesicular 5-HT release in BON cells is 80% compared to previous work in pancreatic beta cells (34%). The fractional release increased from 80% in control BON cells to 87% with 5-HT preincubation and nearly 100% with the combination of 5-HT and the 5HT4 autoreceptor agonist, cisapride. Thus, partial release is the primary mechanism of exocytosis in BON cells, resulting in a variable amount of the vesicular content being released. Factors that control secretion of 5-HT from enterochromaffin cells or BON cells are important as partial release provides a mechanism for development of effective therapeutic strategies to treat gastrointestinal diseases.

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Nano-analysis reveals high fraction of serotonin release during exocytosis from a gut epithelium model cell

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Keywords

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