In vitro allergenicity of cows' milk substitutes

Taraneh Dean, B.R. Adler, F. Ruge, J.O. Warner

Research output: Contribution to journalArticle

Abstract

There are numerous alternatives to cows' milk formula for allergic children. We have investigated the allergenicity of several of these using RAST and RAST inhibition on serum from 16 patients with a known history of cows' milk protein intolerance (CMPI) and 16 atopic controls. A RAST grade of ≥3 for cows' milk was present in all those with CMPI, whilst all the controls gave RAST of ≤1 - Modified cows' milk formula, goats' infant formula, sheep and goats' milk produced similar results to cows' milk. Only two patients had RAST ≥3 for soya milk and the soy/beef hydrolysate gave positive results in only three patients. One had positive RAST to Nutramigen and two to Pregestimil. Of the whey hydrolysates investigated, Pepti-junior gave seven positive RASTs whilst we were unable to hind Alfare to the sepharose in sufficient quantities to interpret the results which were negative in all cases. RAST inhibition data on pooled sera from the same patients agreed with the RAST results. The inhibition curves showed high inhibition with goats', sheep, modified cows' milk formula and the casein formula. AL110 (50%). Soy and soy/beef hydrolysate showed a much lower inhibition pattern. Casein hydrolysates showed low inhibition while the whey hydrolysate produced higher inhibition. We have shown that despite claims of low allergenicity. some of these alternative formulae are antigenically recognized in vitro by some cows' milk intolerant patients.
Original languageEnglish
Pages (from-to)205-210
Number of pages6
JournalClinical and Experimental Allergy
Volume23
Issue number3
Publication statusPublished - 1 Mar 1993

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milk substitutes
allergenicity
milk
hydrolysates
milk allergy
whey
beef
goats
casein hydrolysates
soymilk
ewe milk
goat milk
infant formulas
agarose
casein
sheep

Cite this

Dean, T., Adler, B. R., Ruge, F., & Warner, J. O. (1993). In vitro allergenicity of cows' milk substitutes. Clinical and Experimental Allergy, 23(3), 205-210.
Dean, Taraneh ; Adler, B.R. ; Ruge, F. ; Warner, J.O. / In vitro allergenicity of cows' milk substitutes. In: Clinical and Experimental Allergy. 1993 ; Vol. 23, No. 3. pp. 205-210.
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Dean, T, Adler, BR, Ruge, F & Warner, JO 1993, 'In vitro allergenicity of cows' milk substitutes', Clinical and Experimental Allergy, vol. 23, no. 3, pp. 205-210.

In vitro allergenicity of cows' milk substitutes. / Dean, Taraneh; Adler, B.R.; Ruge, F.; Warner, J.O.

In: Clinical and Experimental Allergy, Vol. 23, No. 3, 01.03.1993, p. 205-210.

Research output: Contribution to journalArticle

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Dean T, Adler BR, Ruge F, Warner JO. In vitro allergenicity of cows' milk substitutes. Clinical and Experimental Allergy. 1993 Mar 1;23(3):205-210.