Identification and characterisation of human aldose 1-epimerase

David J. Timson, Richard J. Reece

Research output: Contribution to journalArticlepeer-review

Abstract

Aldose 1-epimerase or mutarotase (EC 5.1.3.3) is a key enzyme of carbohydrate metabolism catalysing the interconversion of the α- and β-anomers of hexose sugars such as glucose and galactose. We identified an open reading frame in the human genome (BC014916) which has high sequence similarity to previously identified bacterial aldose 1-epimerases. This sequence was cloned into a bacterial expression vector, and expressed and purified from this source. Enzyme assays show that the protein has aldose 1-epimerase activity and exhibits a preference for galactose over glucose. Site-directed mutagenesis confirmed the involvement of three residues involved in catalysis and substrate binding.

Original languageEnglish
Pages (from-to)21-24
Number of pages4
JournalFebs Letters
Volume543
Issue number1-3
DOIs
Publication statusPublished - 22 May 2003

Keywords

  • Carbohydrate metabolism
  • Galactose
  • Galactosemia
  • Glucose
  • Leloir pathway
  • Mutarotase

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