Identification and characterisation of anti - Pseudomonas aeruginosa proteins in mucus of the brown garden snail, Cornu aspersum

Sarah Pitt, J.A. Hawthorne, M. Garcia-Maya, A. Alexandrovich, R.C. Symonds, A. Gunn

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Background
Novel antimicrobial treatments are urgently needed. Previous work has shown that the mucus of the brown garden snail (Cornu aspersum) has antimicrobial properties, in particular against type culture collection strains of Pseudomonas aeruginosa. We hypothesised that it would also be effective against clinical isolates of the bacterium and that investigation of fractions of the mucus would identify one or more proteins with anti-pseudomonal properties, which could be further characterised.
Materials and methods: Mucus was extracted from snails collected from the wild. Antimicrobial activity against laboratory and clinical isolates of Ps. aeruginosa was determined in disc diffusion assays. Mucus was purified using size exclusion chromatography and fractions containing anti-pseudomonal activity identified. Mass spectroscopy and high performance liquid chromatography analysis of these fractions yielded partial peptide sequences. These were used to interrogate an RNA transcriptome generated from whole snails.
Results: Mucus from C. aspersum inhibited growth of type collection strains and clinical isolates of Ps. aeruginosa. Four novel C. aspersum proteins were identified; at least three are likely to have antimicrobial properties. The most interesting is a 37.4 kDa protein whilst smaller proteins, one 17.5 kDa and one 18.6 kDa also appear to have activity against Ps. aeruginosa.
Conclusions: The study has identified novel proteins with antimicrobial properties which could be used to develop treatments for use in human medicine.
Original languageEnglish
Pages (from-to)129-136
Number of pages8
JournalBritish Journal of Biomedical Science
Volume76
Issue number3
DOIs
Publication statusPublished - 17 Jun 2019

Fingerprint

Snails
Mucus
Pseudomonas aeruginosa
Proteins
Protein C
Transcriptome
Gel Chromatography
Mass Spectrometry
High Pressure Liquid Chromatography
Medicine
RNA
Bacteria
Peptides
Growth

Bibliographical note

This is an Accepted Manuscript of an article published by Taylor & Francis Group in British Journal of Biomedical Science on 17/06/2019, available online: http://www.tandfonline.com/10.1080/09674845.2019.1603794

Keywords

  • Antimicrobial
  • Cornu aspersum
  • Pseudomonas aeruginosa
  • lectin
  • mucus
  • transcriptome

Cite this

Pitt, Sarah ; Hawthorne, J.A. ; Garcia-Maya, M. ; Alexandrovich, A. ; Symonds, R.C. ; Gunn, A. / Identification and characterisation of anti - Pseudomonas aeruginosa proteins in mucus of the brown garden snail, Cornu aspersum. In: British Journal of Biomedical Science. 2019 ; Vol. 76, No. 3. pp. 129-136.
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Identification and characterisation of anti - Pseudomonas aeruginosa proteins in mucus of the brown garden snail, Cornu aspersum. / Pitt, Sarah; Hawthorne, J.A.; Garcia-Maya, M.; Alexandrovich, A.; Symonds, R.C.; Gunn, A.

In: British Journal of Biomedical Science, Vol. 76, No. 3, 17.06.2019, p. 129-136.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Identification and characterisation of anti - Pseudomonas aeruginosa proteins in mucus of the brown garden snail, Cornu aspersum

AU - Pitt, Sarah

AU - Hawthorne, J.A.

AU - Garcia-Maya, M.

AU - Alexandrovich, A.

AU - Symonds, R.C.

AU - Gunn, A.

N1 - This is an Accepted Manuscript of an article published by Taylor & Francis Group in British Journal of Biomedical Science on 17/06/2019, available online: http://www.tandfonline.com/10.1080/09674845.2019.1603794

PY - 2019/6/17

Y1 - 2019/6/17

N2 - BackgroundNovel antimicrobial treatments are urgently needed. Previous work has shown that the mucus of the brown garden snail (Cornu aspersum) has antimicrobial properties, in particular against type culture collection strains of Pseudomonas aeruginosa. We hypothesised that it would also be effective against clinical isolates of the bacterium and that investigation of fractions of the mucus would identify one or more proteins with anti-pseudomonal properties, which could be further characterised. Materials and methods: Mucus was extracted from snails collected from the wild. Antimicrobial activity against laboratory and clinical isolates of Ps. aeruginosa was determined in disc diffusion assays. Mucus was purified using size exclusion chromatography and fractions containing anti-pseudomonal activity identified. Mass spectroscopy and high performance liquid chromatography analysis of these fractions yielded partial peptide sequences. These were used to interrogate an RNA transcriptome generated from whole snails.Results: Mucus from C. aspersum inhibited growth of type collection strains and clinical isolates of Ps. aeruginosa. Four novel C. aspersum proteins were identified; at least three are likely to have antimicrobial properties. The most interesting is a 37.4 kDa protein whilst smaller proteins, one 17.5 kDa and one 18.6 kDa also appear to have activity against Ps. aeruginosa.Conclusions: The study has identified novel proteins with antimicrobial properties which could be used to develop treatments for use in human medicine.

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