Treatment of neonatal rat islets of Langerhans with combined cytokines (interleukin-1beta 10(-10) M, tumour necrosis factor-alpha 10(-10) M, interferon-gamma 5 U/ml) led to extensive cell death, which was potentiated by Fas activation with the anti-Fas cytolytic antibody JO2. Pre-treatment with insulin (25 ng/ml) or insulin-like growth factor-1 (10(-8)M) gave only partial protection against cell killing, but prevented the Fas-mediated component. In the absence of cytokine treatment, Fas-mediated killing was not observed.
Bibliographical note© 1998 Federation of European Biochemical Societies.
- Growth factor
- Islet of Langerhans
Harrison, M., Dunger, A. M., Berg, S., Mabley, J., John, N., Green, M. H. L., & Green, I. C. (1998). Growth factor protection against cytokine-induced apoptosis in neonatal rat islets of Langerhans: role of Fas. Febs Letters, 435(2-3), 207-210. https://doi.org/10.1016/S0014-5793(98)01051-5