Development of a novel mass spectrometric technique for studying DNA damage

Tony L. Merrigan; C. Adam Hunniford; David J. Timson; Martin Catney; Robert W. McCullough

doi:10.1042/BST0370905

Development of a novel mass spectrometric technique for studying DNA damage

Tony L. Merrigan, C. Adam Hunniford, David J. Timson, Martin Catney, Robert W. McCullough

Research output: Contribution to journal › Article › peer-review

Abstract

An experimental system, based upon UV and IR laser desorption, has been constructed to enable the production and characterization of neutral biomolecular targets. These targets are to be used for interaction experiments investigating radiation-induced damage to DNA. The viability of the laser-desorption techniques of MALDI (matrix-assisted laser-desorption ionization), SALDI (surface-assisted laser-desorption ionization) and DIOS (desorption/ionization on silicon), for production of these gas targets is discussed in the present paper. Fluorescent dye tagging and LIF (laser-induced fluorescence) imaging has been used to characterize the biomolecular plumes, revealing their spatial density profiles and temporal evolution.

Original language	English
Pages (from-to)	905-909
Number of pages	5
Journal	Biochemical Society Transactions
Volume	37
Issue number	4
DOIs	https://doi.org/10.1042/BST0370905
Publication status	Published - 29 Sept 2009

Keywords

Desorption/ionization on silicon (DIOS)
Laser desorption
Mass spectrometry
Matrix-assisted laser-desorption ionization (MALDI)
Surface-assisted laser-desorption ionization (SALDI)

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10.1042/BST0370905

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title = "Development of a novel mass spectrometric technique for studying DNA damage",

abstract = "An experimental system, based upon UV and IR laser desorption, has been constructed to enable the production and characterization of neutral biomolecular targets. These targets are to be used for interaction experiments investigating radiation-induced damage to DNA. The viability of the laser-desorption techniques of MALDI (matrix-assisted laser-desorption ionization), SALDI (surface-assisted laser-desorption ionization) and DIOS (desorption/ionization on silicon), for production of these gas targets is discussed in the present paper. Fluorescent dye tagging and LIF (laser-induced fluorescence) imaging has been used to characterize the biomolecular plumes, revealing their spatial density profiles and temporal evolution.",

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AU - Merrigan, Tony L.

AU - Hunniford, C. Adam

AU - Timson, David J.

AU - Catney, Martin

AU - McCullough, Robert W.

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AB - An experimental system, based upon UV and IR laser desorption, has been constructed to enable the production and characterization of neutral biomolecular targets. These targets are to be used for interaction experiments investigating radiation-induced damage to DNA. The viability of the laser-desorption techniques of MALDI (matrix-assisted laser-desorption ionization), SALDI (surface-assisted laser-desorption ionization) and DIOS (desorption/ionization on silicon), for production of these gas targets is discussed in the present paper. Fluorescent dye tagging and LIF (laser-induced fluorescence) imaging has been used to characterize the biomolecular plumes, revealing their spatial density profiles and temporal evolution.

KW - Desorption/ionization on silicon (DIOS)

KW - Laser desorption

KW - Mass spectrometry

KW - Matrix-assisted laser-desorption ionization (MALDI)

KW - Surface-assisted laser-desorption ionization (SALDI)

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Development of a novel mass spectrometric technique for studying DNA damage

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