Abstract
Parasitic diseases caused by helminths (worms) represent a major burden on humanity with hundreds of millions of people infected worldwide. However, there are relatively few drugs to treat these diseases, and resistance is emerging to some of these. Therefore, there is a pressing need to characterize proteins from helminths as potential drug targets. Calcium signalling proteins represent attractive targets due to the vital nature of properly regulated calcium-mediated signalling and the presence of unusual calcium-binding proteins in helminths. Here we present methods to characterize these proteins in terms of their ion-binding properties, drug-binding properties, and oligomeric state, including a method to correct for the effects of non-spherical proteins in analytical gel filtration. In addition we present an overview of their recombinant expression and purification and methods to predict their structures.
| Original language | English |
|---|---|
| Title of host publication | Calcium-Binding Proteins of the EF-Hand Superfamily |
| Editors | C. Heizmann |
| Place of Publication | New York |
| Pages | 615-641 |
| Number of pages | 27 |
| Volume | 1929 |
| ISBN (Electronic) | 9781493990306 |
| DOIs | |
| Publication status | Published - 2 Feb 2019 |
Publication series
| Name | Methods in molecular biology (Clifton, N.J.) |
|---|
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Keywords
- Calcium ion
- Cross-linking
- Divalent cation
- Drug binding
- EF-hand
- Fasciola spp.
- Native gel electrophoresis
- Neglected tropical disease
- Protein modelling
- Schistosoma spp.
- Trematodes
Cite this
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Characterization of Calcium-Binding Proteins from Parasitic Worms. / Thomas, Charlotte M.; Timson, David J.
Calcium-Binding Proteins of the EF-Hand Superfamily. ed. / C. Heizmann. Vol. 1929 New York, 2019. p. 615-641 (Methods in molecular biology (Clifton, N.J.)).Research output: Chapter in Book/Conference proceeding with ISSN or ISBN › Chapter › Research › peer-review
TY - CHAP
T1 - Characterization of Calcium-Binding Proteins from Parasitic Worms
AU - Thomas, Charlotte M.
AU - Timson, David J.
PY - 2019/2/2
Y1 - 2019/2/2
N2 - Parasitic diseases caused by helminths (worms) represent a major burden on humanity with hundreds of millions of people infected worldwide. However, there are relatively few drugs to treat these diseases, and resistance is emerging to some of these. Therefore, there is a pressing need to characterize proteins from helminths as potential drug targets. Calcium signalling proteins represent attractive targets due to the vital nature of properly regulated calcium-mediated signalling and the presence of unusual calcium-binding proteins in helminths. Here we present methods to characterize these proteins in terms of their ion-binding properties, drug-binding properties, and oligomeric state, including a method to correct for the effects of non-spherical proteins in analytical gel filtration. In addition we present an overview of their recombinant expression and purification and methods to predict their structures.
AB - Parasitic diseases caused by helminths (worms) represent a major burden on humanity with hundreds of millions of people infected worldwide. However, there are relatively few drugs to treat these diseases, and resistance is emerging to some of these. Therefore, there is a pressing need to characterize proteins from helminths as potential drug targets. Calcium signalling proteins represent attractive targets due to the vital nature of properly regulated calcium-mediated signalling and the presence of unusual calcium-binding proteins in helminths. Here we present methods to characterize these proteins in terms of their ion-binding properties, drug-binding properties, and oligomeric state, including a method to correct for the effects of non-spherical proteins in analytical gel filtration. In addition we present an overview of their recombinant expression and purification and methods to predict their structures.
KW - Calcium ion
KW - Cross-linking
KW - Divalent cation
KW - Drug binding
KW - EF-hand
KW - Fasciola spp.
KW - Native gel electrophoresis
KW - Neglected tropical disease
KW - Protein modelling
KW - Schistosoma spp.
KW - Trematodes
UR - http://www.scopus.com/inward/record.url?scp=85060927018&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-9030-6_39
DO - 10.1007/978-1-4939-9030-6_39
M3 - Chapter
SN - 9781493990290
VL - 1929
T3 - Methods in molecular biology (Clifton, N.J.)
SP - 615
EP - 641
BT - Calcium-Binding Proteins of the EF-Hand Superfamily
A2 - Heizmann, C.
CY - New York
ER -