Characteristics of interfacial water affected by proteins adsorbed on activated carbon

T.A. Alekseeva, N.I. Lebovka, V.M. Gun'ko, V.V. Strashko, Sergey Mikhalovsky

Research output: Contribution to journalArticlepeer-review


The influence of proteins (bovine serum albumin, BSA, and mouse γ-globulin, IgG) physically adsorbed or covalently attached via coupling with N-cyclohexyl-N′-(2-morpholinoethyl) carbodiimide methyl-p-toluenesulfonate, CMC, to the surface of activated carbon SCN (spherical carbon with nitrogen) on the mobility of interfacial water molecules was studied by means of 1H NMR spectroscopy with freezing-out of bulk water at 180<T<273 K. Relaxation processes in the interfacial non-freezing water were investigated measuring transverse time t2 of proton relaxation dependence on the presence of proteins and CMC. The distribution function of activation free energy of relaxation (with a maximum at 20–22 kJ/mol) was calculated for the protein–water–carbon systems using a regularization procedure and the relationships between t2 and the amounts of the interfacial water unfrozen at T<250 K assuming the Arrhenius-type dependence for t2−1 on temperature. The state of unfrozen water in pores of SCN shows that the low temperature relaxation processes occur in narrow pores with half-width X<1.5 nm.
Original languageEnglish
Pages (from-to)333-341
Number of pages9
JournalJournal of Colloid and Interface Science
Issue number2
Publication statusPublished - Oct 2004


  • Activated carbon
  • Microporosity
  • Surface properties
  • 1H NMR spectroscopy
  • Nonfreezable interfacial water
  • Protein immobilization
  • Protein chemisorption


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