Catalytic superoxide scavenging by metal complexes of the calcium chelator EGTA and contrast agent EHPG

A.E.O. Fisher, T.A. Hague, C.L. Clarke, D.P. Naughton

Research output: Contribution to journalArticle

Abstract

Metal ion chelators widely used in experimental protocols and clinical diagnosis are generally assumed to be inert. We previously reported that the ubiquitous chelator EDTA has high levels of superoxide suppressing activity. Here, we report that the common chelators calcium chelator EGTA and contrast agent EHPG have significant activities in suppressing superoxide levels depending on the nature of metal ion chelated. The most active species is Mn(II)-EGTA which exhibited an IC50 value of 0.19 microM for superoxide destruction. In addition, IC50 values for Mn(II)-EHPG and 2Cu(II)-EGTA were 0.69 and 0.60 microM, respectively. In conclusion, Mn(II) and Cu(II) complexes of the common chelators EGTA and EHPG exhibit considerable superoxide scavenging activities. Caution should be employed in their use in biological systems where superoxide has a key role and they may be useful for the development of catalytic anti-oxidants.
Original languageEnglish
Pages (from-to)163-7
Number of pages5
JournalBiochemical and biophysical research communications
Volume323
Issue number1
DOIs
Publication statusPublished - Oct 2004

Keywords

  • Chelator
  • Superoxide dismutase
  • Metal ions
  • Ethylenebis(hydroxyphenylglycine)
  • Ethylenebis(oxyethylenenitrilo)tetraacetic acid

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