This paper studies the incolumn detection performance for chromatography separation. Numerical modeling was performed to investigate the detection efficiency of the electrodes that are suspended within a packed chromatography column. The results show that the density of the silica beads packed within the channel does not hinder the detection ability of the electrode. The findings have been further validated with experimental works.An 8 incolumn electrode array provides the full separation progress traces of the five neurotransmitters and metabolites (adrenalin, dopamine, DOPAC, serotonin and 5HIAA) within the column. From the incolumn detection, better peak resolutions are developed though the separation column as compared to the conventional postcolumn detection. The whole progress of the separation shows that good separation (R > 1) can be obtained at E5 (28.7 mm from column inlet), whereas baseline separation could be obtained at E8 (last incolumn electrode) with R ≥ 1.5 within 60 min. The presented results suggest that the packing materials within the column do not obstruct the efficiency of the electrodes but able to produce a good baseline separation detection as compared to conventional postcolumn detection.
Bibliographical noteThe final publication is available at Springer via http://dx.doi.org/ 10.1007/s10404-015-1561-9
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- School of Applied Sciences - Prof. Clinical and Bioanalytical Chemistry
- Applied Chemical Sciences Research and Enterprise Group
- Centre for Lifelong Health