A rat monoclonal antibody that catalyses the hydrolysis of a nitrophenyl-β-lactam

Elizabeth Ostler, C.J. Dean, N. Barber, M. Valeri, Stuart James, M. Resmini, G. Boucher, N. Romanov, K. Brocklehurst, Gerard Gallacher

Research output: Contribution to journalArticle

Abstract

We report the first example of a monoclonal antibody-catalysed hydrolysis of a β-lactam where the antibodies were generated by a simple transition-state analogue. A rat monoclonal antibody (1/91c/4d/26) generated by using an acyclic 4-nitrophenylphosphate immunogen catalysed the hydrolysis of corresponding 4-nitrophenyl carbonates but, more importantly, also catalysed the hydrolysis of N-(4-nitrophenyl)-azetidinone at pH 8 with kcat=8.7×10−6 s−1 and KM=35 μM. This is the first example of a rat monoclonal catalytic antibody.
Original languageEnglish
Pages (from-to)273-276
Number of pages4
JournalBiochemical and biophysical research communications
Volume299
Issue number2
DOIs
Publication statusPublished - Nov 2002

Keywords

  • Catalytic antibodies
  • β-Lactam
  • Hydrolysis
  • Monoclonal antibodies
  • Abzyme

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