TY - JOUR
T1 - A rapid, non-destructive method for the determination of Staphylococcus epidermidis adhesion to surfaces using quartz crystal resonant sensor technology
AU - Pavey, K.D.
AU - Barnes, Lara-Marie
AU - Hanlon, Geoff
AU - Olliff, C.J.
AU - Ali, Z.
AU - Paul, F.
PY - 2001/11
Y1 - 2001/11
N2 - Aims: To investigate the use of quartz crystal resonant sensor (QCRS) technology to determine the adhesion of Staphylococcus epidermidis to fibronectin-coated surfaces.
Methods and Results: QCRS sensors (14 MHz) with 4 mm gold electrodes were coated with fibronectin and exposed for 15 min to suspensions of Staph. epidermidis ranging in concentration from 1 × 102 to 1 × 106 cfu ml-1. Changes in resonant frequency were recorded and showed a linear relationship with the logarithm of cell concentration over the range tested.
Conclusions: QCRS technology was shown to be a rapid, sensitive and non-destructive method for measuring the adhesion of bacteria to surfaces.
Significance and Impact of the Study: This report demonstrates that QCRS technology has the potential to be used for a range of applications requiring measurement of bacteria on surfaces. In particular, it may be used for the real-time monitoring of bacterial biofilm formation.
AB - Aims: To investigate the use of quartz crystal resonant sensor (QCRS) technology to determine the adhesion of Staphylococcus epidermidis to fibronectin-coated surfaces.
Methods and Results: QCRS sensors (14 MHz) with 4 mm gold electrodes were coated with fibronectin and exposed for 15 min to suspensions of Staph. epidermidis ranging in concentration from 1 × 102 to 1 × 106 cfu ml-1. Changes in resonant frequency were recorded and showed a linear relationship with the logarithm of cell concentration over the range tested.
Conclusions: QCRS technology was shown to be a rapid, sensitive and non-destructive method for measuring the adhesion of bacteria to surfaces.
Significance and Impact of the Study: This report demonstrates that QCRS technology has the potential to be used for a range of applications requiring measurement of bacteria on surfaces. In particular, it may be used for the real-time monitoring of bacterial biofilm formation.
U2 - 10.1046/j.1472-765X.2001.01009.x
DO - 10.1046/j.1472-765X.2001.01009.x
M3 - Article
SN - 1365-2673
VL - 33
SP - 344
EP - 348
JO - Letters in Applied Microbiology
JF - Letters in Applied Microbiology
IS - 5
ER -