A low volume 3D-printed temperature-controllable cuvette for UV visible spectroscopy

Jelena Pisaruka, Marcus Dymond

Research output: Contribution to journalArticle

Abstract

We report the fabrication of a 3D-printed water-heated cuvette that fits into a standard UV visible spectrophotometer. Full 3D-printable designs are provided and 3D-printing conditions have been optimised to provide options to print the cuvette in either acrylonitrile butadiene styrene or polylactic acid polymers, extending the range of solvents that are compatible with the design. We demonstrate the efficacy of the cuvette by determining the critical micelle concentration of sodium dodecyl sulphate at 40°C, the molar extinction coefficients of cobalt nitrate and dsDNA and by reproducing the thermochromic UV visible spectrum of a mixture of cobalt chloride, water and propan-2-ol.
Original languageEnglish
Pages (from-to)52-55
Number of pages4
JournalAnalytical Biochemistry
Volume510
DOIs
Publication statusPublished - 19 Jul 2016

Bibliographical note

© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/

Keywords

  • 3D-printing
  • Critical micelle concentration
  • UV visible spectroscopy
  • Thermochromic spectra
  • Water-heated cuvette

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