AbstractRegulation of transmembrane ion transport is a vital aspect in the maintenance of a healthy organism. To understand how this highly selective process occurs, how it can become impaired and how impairment may be treated, model compounds are useful tools. Several systems are presently being explored but one of the most widely applicable combines a rigid macrocycle, capable of size-based ion recognition, with membrane spanning substituents that allow the target ions to transverse a phospholipid bilayer. The calixarene class of macrocycles is ideally suited to this task. Previous work had shown that oxacalixarenes could act as models for the filters in natural transmembrane ion channels. Nitrogen-containing analogues of these calixarenes, azacalixarenes, were investigated with a view to constructing a chloride transporting system. Synthetic difficulties encountered when introducing lower rim substitutents precluded the use of azacalixarenes and attention turned to 4-t-butylcalix[n]arenes. 4-t-Butylcalix- and arenes were derivatised with a commercial, membrane disrupting surfactant, Triton X-100®, forming compounds designed to form lipid bilayer-spanning, channel-like structures. The ion transporting ability of these, and other bilayer-spanning O-substituted calixarene derivatives, was determined by planar bilayer electrophysiological methods. Results showed that this modular approach to artificial ion channel construction was successful; calixarene derivatives formed transmembrane channels that allowed sodium ions to pass through but not the larger potassium ions.
|Date of Award||Aug 2011|
|Supervisor||Peter Cragg (Supervisor) & Marcus Allen (Supervisor)|
Transmembrane ion transport by calixarenes
Iqbal, K. S. J. (Author). Aug 2011
Student thesis: Doctoral Thesis