Tuberculosis is endemic in the Gambian population, in which the
magnitude of mycobacterial antigen-driven interferon-γ (IFN-γ) response in BCG
vaccinated neonates has been linked to regions on the genome that encode the
RIP2 kinase, the toll-like receptor 4 adapter protein MD-2 and the NF-κB subunit
NF-κB2 by genome-wide linkage analysis.
The receptor interacting protein (RIP2) is an essential kinase downstream
of the nucleotide-binding oligomerization domain-containing protein 1 (NOD1) and
NOD2, both intracellular pattern-recognition receptors for peptidoglycan moieties
that induce activation of NF-κB. To establish the significance of RIP2 kinase
during Mycobacterium tuberculosis infection, RIP2 was depleted in THP-1-
derived macrophages using small interfering RNAs. In the absence of RIP2, THP-
1-derived macrophages secreted significantly reduced levels of the proinflammatory
cytokine IL-1β upon infection with M. tuberculosis.
Date of Award | Mar 2015 |
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Original language | English |
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Awarding Institution | |
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Characterisation of host determinants that influence host-pathogen interaction during infection with
Mycobacterium tuberculosis
Kreutzfeldt, K. M. (Author). Mar 2015
Student thesis: Doctoral Thesis