Natural products have been the source of many of our successful drugs providing us with an
unrivalled chemical diversity combined with drug-like properties. The search for bioactive
compounds can be helped considerably by the phytotherapeutic knowledge held by
indigenous communities. In this study solvent extracts from the bark of a Dalbergia species
and used by a community in Borneo, will be investigated to isolate, identify and
biochemically characterise compounds showing vasorelaxation.
At the core of this study is the hypertensive model, which uses phenylephrine precontracted
rat aortic rings as a bioassay to identify solvent extracts, fractions and sub-fractions that cause
relaxation. These fractions are generated using chromatographic techniques and solvent
systems developed specifically for this purpose. Structural elucidation of the isolated
compounds was undertaken by studying extensive data from UV, MS, 1D and 2D 1H and 13C
NMR spectra. This study also undertook the pharmacological characterisation of the isolated
compounds using the same bioassay together with enzyme and receptor inhibitors to identify
the signalling pathways involved.
|Date of Award||2016|