Pillararenes containing sulfonate fragments have been shown to form supramolecular complexes with therapeutic proteins to facilitate targeted transport with an increased duration of action and enhanced bioavailability. Regioselective synthesis was used to obtain a water-soluble pillararene containing the fluorescent label FITC and nine sulfoethoxy fragments. The pillararene formed complexes with the therapeutic proteins binase, bleomycin, and lysozyme in a 1:2 ratio as demonstrated by UV–vis and fluorescence spectroscopy. The formation of stable spherical nanosized macrocycle/binase complexes with an average particle size of 200 nm was established by dynamic light scattering and transmission electron microscopy. Flow cytometry demonstrated the ability of macrocycle/binase complexes to penetrate into tumor cells where they exhibited significant cytotoxicity towards A549 cells at 10 −5–10 −6 M while maintaining the enzymatic activity of binase.
Bibliographical noteFunding Information:
The work was supported by the Russian Science Foundation (Grant No 17-13-01208).
The investigation of structure of the compounds by NMR spectroscopy was carried out within the framework of the grant of the President of the Russian Federation for state support of leading scientific schools of the Russian Federation (NSh-2499.2020.3).
- A549 cells
- macrocyclic receptors
- Macrocyclic receptors