Two CYP3A-like genes in the marine mussel Mytilus edulis: mRNA expression modulation following short-term exposure to endocrine disruptors

E. Cubero-Leon, A.M. Puinean, P. Labadie, Corina Ciocan, N. Itoh, M. Kishida, M. Osada, C. Minier, E.M. Hill, J.M. Rotchell

Research output: Contribution to journalArticle

Abstract

Members of the vertebrate CYP3A subfamily are involved in the metabolism of steroids and a wide range of xenobiotics. In this study two CYP3A-like mRNAs have been isolated from the mussel (Mytilus edulis), and their seasonal expression profile and modulation by estrogens examined. Sexual dimorphism of CYP3A-like mRNA expression was not observed in mussel gonads of individuals collected throughout a year. Nevertheless, natural variation in gonadal CYP3A-like mRNA expression was observed, with highest levels of CYP3A isoform1 and lowest levels of CYP3A isoform2 mRNA during the maturation and spawning season. Exposure to a 10% sewage treatment works extract did not result in any significant changes in mRNA expression of CYP3A-like. In contrast, exposure to E2 (200 ng/L) and TBT (100 ng/L) significantly down-regulated the expression of CYP3A-like isoform1 but not CYP3A-like isoform2 suggesting differential regulation.
Original languageEnglish
Pages (from-to)32-39
Number of pages8
JournalMarine Environmental Research
Volume74
Publication statusPublished - 31 Mar 2012

Fingerprint

Mytilus edulis
Endocrine Disruptors
Cytochrome P-450 CYP3A
Bivalvia
Messenger RNA
Genes
Gonads
Xenobiotics
Sewage
Sex Characteristics
Vertebrates
Estrogens
Steroids

Keywords

  • Pollution
  • Ecotoxicology
  • Mussel
  • CYP3A

Cite this

Cubero-Leon, E. ; Puinean, A.M. ; Labadie, P. ; Ciocan, Corina ; Itoh, N. ; Kishida, M. ; Osada, M. ; Minier, C. ; Hill, E.M. ; Rotchell, J.M. / Two CYP3A-like genes in the marine mussel Mytilus edulis: mRNA expression modulation following short-term exposure to endocrine disruptors. In: Marine Environmental Research. 2012 ; Vol. 74. pp. 32-39.
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Cubero-Leon, E, Puinean, AM, Labadie, P, Ciocan, C, Itoh, N, Kishida, M, Osada, M, Minier, C, Hill, EM & Rotchell, JM 2012, 'Two CYP3A-like genes in the marine mussel Mytilus edulis: mRNA expression modulation following short-term exposure to endocrine disruptors', Marine Environmental Research, vol. 74, pp. 32-39.

Two CYP3A-like genes in the marine mussel Mytilus edulis: mRNA expression modulation following short-term exposure to endocrine disruptors. / Cubero-Leon, E.; Puinean, A.M.; Labadie, P.; Ciocan, Corina; Itoh, N.; Kishida, M.; Osada, M.; Minier, C.; Hill, E.M.; Rotchell, J.M.

In: Marine Environmental Research, Vol. 74, 31.03.2012, p. 32-39.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Two CYP3A-like genes in the marine mussel Mytilus edulis: mRNA expression modulation following short-term exposure to endocrine disruptors

AU - Cubero-Leon, E.

AU - Puinean, A.M.

AU - Labadie, P.

AU - Ciocan, Corina

AU - Itoh, N.

AU - Kishida, M.

AU - Osada, M.

AU - Minier, C.

AU - Hill, E.M.

AU - Rotchell, J.M.

PY - 2012/3/31

Y1 - 2012/3/31

N2 - Members of the vertebrate CYP3A subfamily are involved in the metabolism of steroids and a wide range of xenobiotics. In this study two CYP3A-like mRNAs have been isolated from the mussel (Mytilus edulis), and their seasonal expression profile and modulation by estrogens examined. Sexual dimorphism of CYP3A-like mRNA expression was not observed in mussel gonads of individuals collected throughout a year. Nevertheless, natural variation in gonadal CYP3A-like mRNA expression was observed, with highest levels of CYP3A isoform1 and lowest levels of CYP3A isoform2 mRNA during the maturation and spawning season. Exposure to a 10% sewage treatment works extract did not result in any significant changes in mRNA expression of CYP3A-like. In contrast, exposure to E2 (200 ng/L) and TBT (100 ng/L) significantly down-regulated the expression of CYP3A-like isoform1 but not CYP3A-like isoform2 suggesting differential regulation.

AB - Members of the vertebrate CYP3A subfamily are involved in the metabolism of steroids and a wide range of xenobiotics. In this study two CYP3A-like mRNAs have been isolated from the mussel (Mytilus edulis), and their seasonal expression profile and modulation by estrogens examined. Sexual dimorphism of CYP3A-like mRNA expression was not observed in mussel gonads of individuals collected throughout a year. Nevertheless, natural variation in gonadal CYP3A-like mRNA expression was observed, with highest levels of CYP3A isoform1 and lowest levels of CYP3A isoform2 mRNA during the maturation and spawning season. Exposure to a 10% sewage treatment works extract did not result in any significant changes in mRNA expression of CYP3A-like. In contrast, exposure to E2 (200 ng/L) and TBT (100 ng/L) significantly down-regulated the expression of CYP3A-like isoform1 but not CYP3A-like isoform2 suggesting differential regulation.

KW - Pollution

KW - Ecotoxicology

KW - Mussel

KW - CYP3A

M3 - Article

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JO - Marine Environmental Research

JF - Marine Environmental Research

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