Translational control plays an important role in the adaptive heat-shock response of Streptomyces coelicolor

Giselda Bucca, Radhika Pothi, Andrew Hesketh, Carla Moller-Levet, David A. Hodgson, Emma E. Laing, Graham R. Stewart, Colin Smith

Research output: Contribution to journalArticle

Abstract

Stress-induced adaptations require multiple levels of regulation in all organisms to repair cellular damage. In the present study we evaluated the genome-wide transcriptional and translational changes following heat stress exposure in the soil-dwelling model acti- nomycete bacterium, Streptomyces coelicolor. The combined analysis revealed an unprecedented level of translational control of gene expression, deduced through polysome profiling, in addition to transcrip- tional changes. Our data show little correlation be- tween the transcriptome and ‘translatome’; while an obvious downward trend in genome wide transcrip- tion was observed, polysome associated transcripts following heat-shock showed an opposite upward trend. A handful of key protein players, including the major molecular chaperones and proteases were highly induced at both the transcriptional and trans- lational level following heat-shock, a phenomenon known as ‘potentiation’. Many other transcripts en- coding cold-shock proteins, ABC-transporter sys- tems, multiple transcription factors were more highly polysome-associated following heat stress; interest- ingly, these protein families were not induced at the transcriptional level and therefore were not previ- ously identified as part of the stress response. Thus, stress coping mechanisms at the level of gene ex- pression in this bacterium go well beyond the induc- tion of a relatively small number of molecular chap- erones and proteases in order to ensure cellular sur-vival at non-physiological temperatures.
Original languageEnglish
Pages (from-to)5692–5703
JournalNucleic Acids Research
Volume46
Issue number11
DOIs
Publication statusPublished - 9 May 2018

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Streptomyces coelicolor
Heat-Shock Response
Polyribosomes
Hot Temperature
Molecular Chaperones
Shock
Peptide Hydrolases
Cold Shock Proteins and Peptides
Genome
Bacteria
Gene Expression
ATP-Binding Cassette Transporters
Polysorbates
Actinobacteria
Transcriptome
Proteins
Transcription Factors
Soil
Temperature

Bibliographical note

© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this

Bucca, Giselda ; Pothi, Radhika ; Hesketh, Andrew ; Moller-Levet, Carla ; Hodgson, David A. ; Laing, Emma E. ; Stewart, Graham R. ; Smith, Colin. / Translational control plays an important role in the adaptive heat-shock response of Streptomyces coelicolor. In: Nucleic Acids Research. 2018 ; Vol. 46, No. 11. pp. 5692–5703.
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Bucca, G, Pothi, R, Hesketh, A, Moller-Levet, C, Hodgson, DA, Laing, EE, Stewart, GR & Smith, C 2018, 'Translational control plays an important role in the adaptive heat-shock response of Streptomyces coelicolor', Nucleic Acids Research, vol. 46, no. 11, pp. 5692–5703. https://doi.org/10.1093/nar/gky335

Translational control plays an important role in the adaptive heat-shock response of Streptomyces coelicolor. / Bucca, Giselda; Pothi, Radhika; Hesketh, Andrew; Moller-Levet, Carla; Hodgson, David A.; Laing, Emma E.; Stewart, Graham R.; Smith, Colin.

In: Nucleic Acids Research, Vol. 46, No. 11, 09.05.2018, p. 5692–5703.

Research output: Contribution to journalArticle

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AU - Hodgson, David A.

AU - Laing, Emma E.

AU - Stewart, Graham R.

AU - Smith, Colin

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