Topical administration of a novel nitric oxide donor, linear polyethylenimine-nitric oxide/nucleophile adduct (DS1), selectively increases vaginal blood flow in anesthetized rats

P. Pacher, Jon Mabley, L. Liaudet, O.V. Evgenov, G.J. Southan, G.E. Abdelkarim, C. Szabo, A.L. Salzman

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The aim of the present study was to test the effects of a topical administration of a novel nitric oxide donor, linear polyethylenimine-nitric oxide/nucleophile adduct (DS1), on vaginal blood flow and hemodynamics in rats. Laser Doppler flowmetry was used to measure blood flow changes following topical application of DS1 (0.3 or 1.5 mg in 0.15 ml saline) into the vagina of anesthetized Wistar rats. In vivo hemodynamic parameters were measured with Millar-tip-catheter placed in the left ventricle. DS1 (1.5 mg) increased vaginal blood flow by 191±24, 226±22 and 166±23% of the baseline value (at 5, 15 and 30 min, respectively, after application) without affecting systemic blood pressure, heart rate and cardiac function. The increased vaginal blood flow following DS1 application returned to baseline between 45 and 60 min. Thus, topical application of nitric oxide donors such as DS1 may be useful for the treatment of female sexual dysfunction that develops due to an impairment of local blood flow supply to the vaginal tissue.
Original languageEnglish
Pages (from-to)461-464
Number of pages4
JournalInternational Journal of Impotence
Volume15
Issue number6
DOIs
Publication statusPublished - Dec 2003

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Polyethyleneimine
Topical Administration
Nitric Oxide Donors
Nitric Oxide
Hemodynamics
Laser-Doppler Flowmetry
Vagina
Heart Ventricles
Wistar Rats
Catheters
Heart Rate
Blood Pressure

Cite this

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title = "Topical administration of a novel nitric oxide donor, linear polyethylenimine-nitric oxide/nucleophile adduct (DS1), selectively increases vaginal blood flow in anesthetized rats",
abstract = "The aim of the present study was to test the effects of a topical administration of a novel nitric oxide donor, linear polyethylenimine-nitric oxide/nucleophile adduct (DS1), on vaginal blood flow and hemodynamics in rats. Laser Doppler flowmetry was used to measure blood flow changes following topical application of DS1 (0.3 or 1.5 mg in 0.15 ml saline) into the vagina of anesthetized Wistar rats. In vivo hemodynamic parameters were measured with Millar-tip-catheter placed in the left ventricle. DS1 (1.5 mg) increased vaginal blood flow by 191±24, 226±22 and 166±23{\%} of the baseline value (at 5, 15 and 30 min, respectively, after application) without affecting systemic blood pressure, heart rate and cardiac function. The increased vaginal blood flow following DS1 application returned to baseline between 45 and 60 min. Thus, topical application of nitric oxide donors such as DS1 may be useful for the treatment of female sexual dysfunction that develops due to an impairment of local blood flow supply to the vaginal tissue.",
author = "P. Pacher and Jon Mabley and L. Liaudet and O.V. Evgenov and G.J. Southan and G.E. Abdelkarim and C. Szabo and A.L. Salzman",
year = "2003",
month = "12",
doi = "10.1038/sj.ijir.3901045",
language = "English",
volume = "15",
pages = "461--464",
journal = "International Journal of Impotence",
issn = "1476-5489",
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}

Topical administration of a novel nitric oxide donor, linear polyethylenimine-nitric oxide/nucleophile adduct (DS1), selectively increases vaginal blood flow in anesthetized rats. / Pacher, P.; Mabley, Jon; Liaudet, L.; Evgenov, O.V.; Southan, G.J.; Abdelkarim, G.E.; Szabo, C.; Salzman, A.L.

In: International Journal of Impotence, Vol. 15, No. 6, 12.2003, p. 461-464.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Topical administration of a novel nitric oxide donor, linear polyethylenimine-nitric oxide/nucleophile adduct (DS1), selectively increases vaginal blood flow in anesthetized rats

AU - Pacher, P.

AU - Mabley, Jon

AU - Liaudet, L.

AU - Evgenov, O.V.

AU - Southan, G.J.

AU - Abdelkarim, G.E.

AU - Szabo, C.

AU - Salzman, A.L.

PY - 2003/12

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N2 - The aim of the present study was to test the effects of a topical administration of a novel nitric oxide donor, linear polyethylenimine-nitric oxide/nucleophile adduct (DS1), on vaginal blood flow and hemodynamics in rats. Laser Doppler flowmetry was used to measure blood flow changes following topical application of DS1 (0.3 or 1.5 mg in 0.15 ml saline) into the vagina of anesthetized Wistar rats. In vivo hemodynamic parameters were measured with Millar-tip-catheter placed in the left ventricle. DS1 (1.5 mg) increased vaginal blood flow by 191±24, 226±22 and 166±23% of the baseline value (at 5, 15 and 30 min, respectively, after application) without affecting systemic blood pressure, heart rate and cardiac function. The increased vaginal blood flow following DS1 application returned to baseline between 45 and 60 min. Thus, topical application of nitric oxide donors such as DS1 may be useful for the treatment of female sexual dysfunction that develops due to an impairment of local blood flow supply to the vaginal tissue.

AB - The aim of the present study was to test the effects of a topical administration of a novel nitric oxide donor, linear polyethylenimine-nitric oxide/nucleophile adduct (DS1), on vaginal blood flow and hemodynamics in rats. Laser Doppler flowmetry was used to measure blood flow changes following topical application of DS1 (0.3 or 1.5 mg in 0.15 ml saline) into the vagina of anesthetized Wistar rats. In vivo hemodynamic parameters were measured with Millar-tip-catheter placed in the left ventricle. DS1 (1.5 mg) increased vaginal blood flow by 191±24, 226±22 and 166±23% of the baseline value (at 5, 15 and 30 min, respectively, after application) without affecting systemic blood pressure, heart rate and cardiac function. The increased vaginal blood flow following DS1 application returned to baseline between 45 and 60 min. Thus, topical application of nitric oxide donors such as DS1 may be useful for the treatment of female sexual dysfunction that develops due to an impairment of local blood flow supply to the vaginal tissue.

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