The interaction of nanostructured biomaterials with human cell cultures. The choice of cell cultures for use as biocompatability probes

S.Elizabeth James, Matthew Illsley, Stuart James, E. Mendoza, S.R.P. Silva, P. Vadgama, Paul Tomlins, Sergey Mikhalovsky

Research output: Chapter in Book/Conference proceeding with ISSN or ISBNChapter

Abstract

The study of the interaction of cells with nanoparticles or nanosurfaces is increasingly being performed using in vitro methods, largely with immortal cell lines. Immortal cell lines may behave differently from cells initially isolated from living tissue in these assays. Thus we have established a cell bank of primary human cell cultures stored at different stages from initial isolation through to senescence. We have examined the effects of culture age oil proliferation upon carbon nanotube arrays, and adhesion to polystyrene surfaces. These data have been compared with an equivalent telomerase transformed line, and a commercially available line. Initial results suggest very large errors can be introduced into nanotoxicity and adhesion assays by inappropriate use of cell strains and lines.
Original languageEnglish
Title of host publicationSurface chemistry in biomedical and environmental science
EditorsJ.P. Blitz, V.M. Gun'ko
Place of PublicationNetherlands
PublisherSpringer
Pages205-214
Number of pages10
Volume228
ISBN (Electronic)9781402047411
ISBN (Print)9781402047398
DOIs
Publication statusPublished - 31 Dec 2006

Keywords

  • biocompatibility
  • cell culture
  • nanotubes
  • telomerase

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    James, S. E., Illsley, M., James, S., Mendoza, E., Silva, S. R. P., Vadgama, P., Tomlins, P., & Mikhalovsky, S. (2006). The interaction of nanostructured biomaterials with human cell cultures. The choice of cell cultures for use as biocompatability probes. In J. P. Blitz, & V. M. Gun'ko (Eds.), Surface chemistry in biomedical and environmental science (Vol. 228, pp. 205-214). Springer. https://doi.org/10.1007/1-4020-4741-X_18