Individual Streptomyces species have the genetic potential to produce a diverse array ofnatural products of commercial, medical and veterinary interest. However, these products are often not detectable under laboratory culture conditions. To harness their full biosynthetic potential, it is important to develop a detailed understanding of the regulatory networks that orchestrate their metabolism. Here we integrate nucleotide resolution genome-scale measurements of the transcriptome and translatome of Streptomyces coelicolor, the model antibiotic-producing actinomycete. Our systematic study determines 3,570 transcription start sites and identiﬁes 230 small RNAs and a considerable proportion (~21%) of leaderless mRNAs; this enables deduction of genome-wide promoter architecture. Ribosome proﬁling reveals that the translation efﬁciency of secondary metabolic genes is negatively correlated with transcription and that several key antibiotic regulatory genes are translationally induced at transition growth phase. These ﬁndings might facilitate the design of new approaches toantibiotic discovery and development.
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- Biological sciences
- Systems biology