The dynamic transcriptional and translational landscape of the model antibiotic producer Streptomyces coelicolor A3(2)

Yujin Jeong, Ji-Nu Kim, Min Woo Kim, Giselda Bucca, Suhyung Cho, Yeo Joon Yoon, Byung-Gee Kim, Jung-Hye Roe, Sun Chang Kim, Colin Smith, Byung-Kwan Cho

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Abstract

Individual Streptomyces species have the genetic potential to produce a diverse array ofnatural products of commercial, medical and veterinary interest. However, these products are often not detectable under laboratory culture conditions. To harness their full biosynthetic potential, it is important to develop a detailed understanding of the regulatory networks that orchestrate their metabolism. Here we integrate nucleotide resolution genome-scale measurements of the transcriptome and translatome of Streptomyces coelicolor, the model antibiotic-producing actinomycete. Our systematic study determines 3,570 transcription start sites and identifies 230 small RNAs and a considerable proportion (~21%) of leaderless mRNAs; this enables deduction of genome-wide promoter architecture. Ribosome profiling reveals that the translation efficiency of secondary metabolic genes is negatively correlated with transcription and that several key antibiotic regulatory genes are translationally induced at transition growth phase. These findings might facilitate the design of new approaches toantibiotic discovery and development.
Original languageEnglish
Article number 11605
JournalNature Communications
Volume7
DOIs
Publication statusPublished - 2 Jun 2016

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Keywords

  • Biological sciences
  • Genetics
  • Microbiology
  • Systems biology

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    Jeong, Y., Kim, J-N., Kim, M. W., Bucca, G., Cho, S., Yoon, Y. J., Kim, B-G., Roe, J-H., Kim, S. C., Smith, C., & Cho, B-K. (2016). The dynamic transcriptional and translational landscape of the model antibiotic producer Streptomyces coelicolor A3(2). Nature Communications, 7, [ 11605]. https://doi.org/10.1038/ncomms11605