The dynamic transcriptional and translational landscape of the model antibiotic producer Streptomyces coelicolor A3(2)

Yujin Jeong; Ji-Nu Kim; Min Woo Kim; Giselda Bucca; Suhyung Cho; Yeo Joon Yoon; Byung-Gee Kim; Jung-Hye Roe; Sun Chang Kim; Colin Smith; Byung-Kwan Cho

doi:10.1038/ncomms11605

The dynamic transcriptional and translational landscape of the model antibiotic producer Streptomyces coelicolor A3(2)

Yujin Jeong, Ji-Nu Kim, Min Woo Kim, Giselda Bucca, Suhyung Cho, Yeo Joon Yoon, Byung-Gee Kim, Jung-Hye Roe, Sun Chang Kim, Colin Smith, Byung-Kwan Cho

University of Brighton

Research output: Contribution to journal › Article › peer-review

Abstract

Individual Streptomyces species have the genetic potential to produce a diverse array ofnatural products of commercial, medical and veterinary interest. However, these products are often not detectable under laboratory culture conditions. To harness their full biosynthetic potential, it is important to develop a detailed understanding of the regulatory networks that orchestrate their metabolism. Here we integrate nucleotide resolution genome-scale measurements of the transcriptome and translatome of Streptomyces coelicolor, the model antibiotic-producing actinomycete. Our systematic study determines 3,570 transcription start sites and identiﬁes 230 small RNAs and a considerable proportion (~21%) of leaderless mRNAs; this enables deduction of genome-wide promoter architecture. Ribosome proﬁling reveals that the translation efﬁciency of secondary metabolic genes is negatively correlated with transcription and that several key antibiotic regulatory genes are translationally induced at transition growth phase. These ﬁndings might facilitate the design of new approaches toantibiotic discovery and development.

Original language	English
Article number	11605
Pages (from-to)	1-11
Number of pages	11
Journal	Nature Communications
Volume	7
DOIs	https://doi.org/10.1038/ncomms11605
Publication status	Published - 2 Jun 2016

Bibliographical note

This work is licensed under a Creative Commons Attribution 4.0
International License. The images or other third party material in this
article are included in the article’s Creative Commons license, unless indicated otherwise
in the credit line; if the material is not included under the Creative Commons license,
users will need to obtain permission from the license holder to reproduce the material.
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Keywords

Biological sciences
Genetics
Microbiology
Systems biology

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title = "The dynamic transcriptional and translational landscape of the model antibiotic producer Streptomyces coelicolor A3(2)",

abstract = "Individual Streptomyces species have the genetic potential to produce a diverse array ofnatural products of commercial, medical and veterinary interest. However, these products are often not detectable under laboratory culture conditions. To harness their full biosynthetic potential, it is important to develop a detailed understanding of the regulatory networks that orchestrate their metabolism. Here we integrate nucleotide resolution genome-scale measurements of the transcriptome and translatome of Streptomyces coelicolor, the model antibiotic-producing actinomycete. Our systematic study determines 3,570 transcription start sites and identiﬁes 230 small RNAs and a considerable proportion (~21%) of leaderless mRNAs; this enables deduction of genome-wide promoter architecture. Ribosome proﬁling reveals that the translation efﬁciency of secondary metabolic genes is negatively correlated with transcription and that several key antibiotic regulatory genes are translationally induced at transition growth phase. These ﬁndings might facilitate the design of new approaches toantibiotic discovery and development.",

keywords = "Biological sciences, Genetics, Microbiology, Systems biology",

author = "Yujin Jeong and Ji-Nu Kim and Kim, {Min Woo} and Giselda Bucca and Suhyung Cho and Yoon, {Yeo Joon} and Byung-Gee Kim and Jung-Hye Roe and Kim, {Sun Chang} and Colin Smith and Byung-Kwan Cho",

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The dynamic transcriptional and translational landscape of the model antibiotic producer Streptomyces coelicolor A3(2)

Abstract

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Keywords

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