Susceptibility of microcosm subgingival dental plaques to lethal photosensitization

Iain Allan, J.F. O'Neill, C.K. Hope

Research output: Contribution to journalArticle

Abstract

Photodynamic therapy (PDT) offers potential as a non-invasive treatment of periodontal disease. In this study, microcosm biofilmswere grown in vitro under conditions designed to mimic subgingival plaques typically found in patients with periodontitis. To investigate potential PDT modalities, biofilms were exposed to light from a helium/neon laser in conjunction with a photosensitizer, toluidine blue O (TBO), at varying output and concentration, respectively. To determine cytotoxic effects, viability profiling was undertaken on whole biofilms using standard plating methods, and on horizontal cross-sections of biofilms using confocal laser-scanning microscopy (CLSM) in conjunction with a differential viability stain. A light energy dose of 94.5 J in combination with 81.7 µM TBO was found to be optimal, achieving significant kills of over 97%. CLSM enabled visualization of the effects of PDT in three dimensions. Viability profiling of the CLSM images revealed that lethal photosensitization was most effective in the upper layers of biofilm. PDT was found to reduce the viability of subgingivally modelled plaques in vitro by a magnitude similar to that of chlorhexidine digluconate, which is commonly used to treat periodontal disease. The findings of this study indicate that PDT may be an effective alternative to conventional modalities in the treatment of periodontal disease.
Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalBiofilms
DOIs
Publication statusPublished - 2007

Fingerprint

Photosensitivity Disorders
Dental Plaque
Photochemotherapy
Biofilms
Periodontal Diseases
Confocal Microscopy
Tolonium Chloride
Neon
Light
Photosensitizing Agents
Gas Lasers
Periodontitis
Coloring Agents
Therapeutics

Bibliographical note

© 2007 Cambridge University Press

Keywords

  • Lethal photosensitisation
  • Subgingival plaque
  • Oral Biofilm
  • Constant-depth film fermenter
  • Photodynamic therapy

Cite this

@article{c60a1f5d156845b9ba8f0ae1a13e9e5b,
title = "Susceptibility of microcosm subgingival dental plaques to lethal photosensitization",
abstract = "Photodynamic therapy (PDT) offers potential as a non-invasive treatment of periodontal disease. In this study, microcosm biofilmswere grown in vitro under conditions designed to mimic subgingival plaques typically found in patients with periodontitis. To investigate potential PDT modalities, biofilms were exposed to light from a helium/neon laser in conjunction with a photosensitizer, toluidine blue O (TBO), at varying output and concentration, respectively. To determine cytotoxic effects, viability profiling was undertaken on whole biofilms using standard plating methods, and on horizontal cross-sections of biofilms using confocal laser-scanning microscopy (CLSM) in conjunction with a differential viability stain. A light energy dose of 94.5 J in combination with 81.7 µM TBO was found to be optimal, achieving significant kills of over 97{\%}. CLSM enabled visualization of the effects of PDT in three dimensions. Viability profiling of the CLSM images revealed that lethal photosensitization was most effective in the upper layers of biofilm. PDT was found to reduce the viability of subgingivally modelled plaques in vitro by a magnitude similar to that of chlorhexidine digluconate, which is commonly used to treat periodontal disease. The findings of this study indicate that PDT may be an effective alternative to conventional modalities in the treatment of periodontal disease.",
keywords = "Lethal photosensitisation, Subgingival plaque, Oral Biofilm, Constant-depth film fermenter, Photodynamic therapy",
author = "Iain Allan and J.F. O'Neill and C.K. Hope",
note = "{\circledC} 2007 Cambridge University Press",
year = "2007",
doi = "10.1017/S1479050507002219",
language = "English",
pages = "1--7",
journal = "Biofilms",
issn = "1479-0505",

}

Susceptibility of microcosm subgingival dental plaques to lethal photosensitization. / Allan, Iain; O'Neill, J.F.; Hope, C.K.

In: Biofilms, 2007, p. 1-7.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Susceptibility of microcosm subgingival dental plaques to lethal photosensitization

AU - Allan, Iain

AU - O'Neill, J.F.

AU - Hope, C.K.

N1 - © 2007 Cambridge University Press

PY - 2007

Y1 - 2007

N2 - Photodynamic therapy (PDT) offers potential as a non-invasive treatment of periodontal disease. In this study, microcosm biofilmswere grown in vitro under conditions designed to mimic subgingival plaques typically found in patients with periodontitis. To investigate potential PDT modalities, biofilms were exposed to light from a helium/neon laser in conjunction with a photosensitizer, toluidine blue O (TBO), at varying output and concentration, respectively. To determine cytotoxic effects, viability profiling was undertaken on whole biofilms using standard plating methods, and on horizontal cross-sections of biofilms using confocal laser-scanning microscopy (CLSM) in conjunction with a differential viability stain. A light energy dose of 94.5 J in combination with 81.7 µM TBO was found to be optimal, achieving significant kills of over 97%. CLSM enabled visualization of the effects of PDT in three dimensions. Viability profiling of the CLSM images revealed that lethal photosensitization was most effective in the upper layers of biofilm. PDT was found to reduce the viability of subgingivally modelled plaques in vitro by a magnitude similar to that of chlorhexidine digluconate, which is commonly used to treat periodontal disease. The findings of this study indicate that PDT may be an effective alternative to conventional modalities in the treatment of periodontal disease.

AB - Photodynamic therapy (PDT) offers potential as a non-invasive treatment of periodontal disease. In this study, microcosm biofilmswere grown in vitro under conditions designed to mimic subgingival plaques typically found in patients with periodontitis. To investigate potential PDT modalities, biofilms were exposed to light from a helium/neon laser in conjunction with a photosensitizer, toluidine blue O (TBO), at varying output and concentration, respectively. To determine cytotoxic effects, viability profiling was undertaken on whole biofilms using standard plating methods, and on horizontal cross-sections of biofilms using confocal laser-scanning microscopy (CLSM) in conjunction with a differential viability stain. A light energy dose of 94.5 J in combination with 81.7 µM TBO was found to be optimal, achieving significant kills of over 97%. CLSM enabled visualization of the effects of PDT in three dimensions. Viability profiling of the CLSM images revealed that lethal photosensitization was most effective in the upper layers of biofilm. PDT was found to reduce the viability of subgingivally modelled plaques in vitro by a magnitude similar to that of chlorhexidine digluconate, which is commonly used to treat periodontal disease. The findings of this study indicate that PDT may be an effective alternative to conventional modalities in the treatment of periodontal disease.

KW - Lethal photosensitisation

KW - Subgingival plaque

KW - Oral Biofilm

KW - Constant-depth film fermenter

KW - Photodynamic therapy

U2 - 10.1017/S1479050507002219

DO - 10.1017/S1479050507002219

M3 - Article

SP - 1

EP - 7

JO - Biofilms

JF - Biofilms

SN - 1479-0505

ER -