Survival of Clostridium botulinum in hot-fill meals

S. Rodgers

Research output: Contribution to journalArticle

Abstract

Sous-vide technology poses a risk of botulism. Twenty-six catering and retail cook-chill meals were challenged with non-proteolytic Clostridium botulinum (10(3) spores/g) and incubated for 10 days at 10 degrees celsius. C. botulinum populations were enumerated on salicin tryptic soy agar and background microflora - on plate count agar. Botulinal toxin was detected using the enzyme-linked immunosassay. Only ten of the products supported the active growth of this pathogen. C. botulinum populations were static in another ten products which had a low PH except for two vegetable-based soups. In the remaining six products, C. botulinum populations reduced to undetectable levels. Although the predictive models described the general growth pattern of C. botulinum in the products supporting the active growth and the products with low PH values, they did not predict the spontaneous decline of this pathogen and the static populations in high PH vegetable soups.
Original languageEnglish
Pages (from-to)69-79
Number of pages11
JournalFood Service Technology
Volume2
Issue number2
Publication statusPublished - 2002

Fingerprint

Clostridium botulinum
soups
agar
vegetables
sous vide
catering
botulism
pathogens
plate count
toxins
spores
microorganisms
enzymes

Keywords

  • sous-vide
  • hot-fill
  • C. botulinum
  • predictive models

Cite this

Rodgers, S. / Survival of Clostridium botulinum in hot-fill meals. In: Food Service Technology. 2002 ; Vol. 2, No. 2. pp. 69-79.
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Survival of Clostridium botulinum in hot-fill meals. / Rodgers, S.

In: Food Service Technology, Vol. 2, No. 2, 2002, p. 69-79.

Research output: Contribution to journalArticle

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AB - Sous-vide technology poses a risk of botulism. Twenty-six catering and retail cook-chill meals were challenged with non-proteolytic Clostridium botulinum (10(3) spores/g) and incubated for 10 days at 10 degrees celsius. C. botulinum populations were enumerated on salicin tryptic soy agar and background microflora - on plate count agar. Botulinal toxin was detected using the enzyme-linked immunosassay. Only ten of the products supported the active growth of this pathogen. C. botulinum populations were static in another ten products which had a low PH except for two vegetable-based soups. In the remaining six products, C. botulinum populations reduced to undetectable levels. Although the predictive models described the general growth pattern of C. botulinum in the products supporting the active growth and the products with low PH values, they did not predict the spontaneous decline of this pathogen and the static populations in high PH vegetable soups.

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