Surface and interface analysis of functional proteins and peptides

Sarah Fearn, Santanu Ray

Research output: Chapter in Book/Conference proceeding with ISSN or ISBNChapter

Abstract

Understanding proteins and peptides at the surfaces and interfaces are key to many biological processes and biotechnological development. Revealing quantitative and qualitative details of biomolecules at the interface not only provides further knowledge on these proteins and peptides, but also helps in improvement of life by providing rationale of new and efficient drug invention and biosensor device development. In this current chapter, some of these major analytical techniques used to characterise proteins and peptides are investigated, emphasising the basic theoretical principle of these techniques and some example applications. All these methods have various strengths and weaknesses. A short discussion on the usefulness of these techniques is given, to find highlight the most appropriate technique for the system under investigation. A comparison of the sensitivity between some of these techniques is also given where appropriate. Individual sections are arranged for each analytical technique and a future perspective is presented at the end.
Original languageEnglish
Title of host publicationAmino Acids, Peptides and Proteins
EditorsMaxim Ryadnov, Ferenc Hudecz
Place of PublicationCambridge, UK
PublisherThe Royal Society of Chemistry
Pages225-272
Number of pages48
Volume41
ISBN (Electronic)9781782626619
ISBN (Print)9781782625377
DOIs
Publication statusPublished - 25 Nov 2016

Bibliographical note

© The Royal Society of Chemistry 2017

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    Fearn, S., & Ray, S. (2016). Surface and interface analysis of functional proteins and peptides. In M. Ryadnov, & F. Hudecz (Eds.), Amino Acids, Peptides and Proteins (Vol. 41, pp. 225-272). The Royal Society of Chemistry. https://doi.org/10.1039/9781782626619-00225