Reference gene selection for qPCR in mussel, Mytilus edulis, during gametogenesis and exogenous estrogen exposure

E. Cubero-Leon; Corina Ciocan; C. Minier; J.M. Rotchell

doi:10.1007/s11356-012-0772-9

Reference gene selection for qPCR in mussel, Mytilus edulis, during gametogenesis and exogenous estrogen exposure

E. Cubero-Leon
, Corina Ciocan
, C. Minier
, J.M. Rotchell

University of Brighton

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of this study is to develop a normalization method for real-time PCR data by analyzing the most stably expressed control genes in mussel (Mytilus edulis) reproductive tissue.

Original language	English
Pages (from-to)	2728-2733
Number of pages	6
Journal	Environmental Science and Pollution Research
Volume	19
Issue number	7
DOIs	https://doi.org/10.1007/s11356-012-0772-9
Publication status	Published - 31 Aug 2012

Keywords

Mytilus edulis
Reference genes
Normalization
Endocrine disruption
Real-time PCR
Toxicology

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title = "Reference gene selection for qPCR in mussel, Mytilus edulis, during gametogenesis and exogenous estrogen exposure",

abstract = "The aim of this study is to develop a normalization method for real-time PCR data by analyzing the most stably expressed control genes in mussel (Mytilus edulis) reproductive tissue.",

keywords = "Mytilus edulis, Reference genes, Normalization, Endocrine disruption, Real-time PCR, Toxicology",

author = "E. Cubero-Leon and Corina Ciocan and C. Minier and J.M. Rotchell",

year = "2012",

month = aug,

day = "31",

doi = "10.1007/s11356-012-0772-9",

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T1 - Reference gene selection for qPCR in mussel, Mytilus edulis, during gametogenesis and exogenous estrogen exposure

AU - Cubero-Leon, E.

AU - Ciocan, Corina

AU - Minier, C.

AU - Rotchell, J.M.

PY - 2012/8/31

Y1 - 2012/8/31

N2 - The aim of this study is to develop a normalization method for real-time PCR data by analyzing the most stably expressed control genes in mussel (Mytilus edulis) reproductive tissue.

AB - The aim of this study is to develop a normalization method for real-time PCR data by analyzing the most stably expressed control genes in mussel (Mytilus edulis) reproductive tissue.

KW - Mytilus edulis

KW - Reference genes

KW - Normalization

KW - Endocrine disruption

KW - Real-time PCR

KW - Toxicology

U2 - 10.1007/s11356-012-0772-9

DO - 10.1007/s11356-012-0772-9

M3 - Article

SN - 0944-1344

VL - 19

SP - 2728

EP - 2733

JO - Environmental Science and Pollution Research

JF - Environmental Science and Pollution Research

IS - 7

ER -

Reference gene selection for qPCR in mussel, Mytilus edulis, during gametogenesis and exogenous estrogen exposure

Abstract

Keywords

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