Presence of bacteria and bacteriophages in full-scale trickling filters and an aerated constructed wetland

Alexandros Stefanakis, Marjorie Bardiau, Diogo Gomes Da Silva, F. Couceiro, J.B. Williams, Huw Taylor

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Aerated Constructed Wetlands are a state-of-the-art design that provides a different physical and chemical environment (compared to traditional passive wetland designs) for the wastewater treatment processes and, thus, may
have different pathogen removal characteristics. In order to establish the fate of bacterial and viral indicators, a field studywas carried out at a Sewage Treatment Works (STW) in the UK (serving 20,000 pe). The STWconsists of primary
and secondary sedimentation tanks and tricklingfilters (TF) as the biological stage.Alarge (1,160m2) pilot aerated Vertical Flow Constructed Wetland (AVFCW) was constructed at the STW as tertiary stage receiving ¼ of the total flow
rate, i.e., 1250m3/day. Effluent quality of the AVFCW complied with national and international standards for environmental discharge and reuse. For the first time, two sets of bacterial (Faecal coliforms, E.coli and intestinal enterococci)
and viral indicators (Somatic coliphages, F-RNA specific bacteriophages and human-specific B. fragilis GB124 phages) were simultaneously investigated in an AVFCW and TF. High elimination rates were detected (up to 3.7 and 2.2 log reduction for bacteria indicators and phages, respectively) and strong correlations between the two sets were found. The superior efficiency of the aerated ConstructedWetlands inmicrobiological contamination removal compared to passive wetland systems was established for the first time, whichmay have implications for process selection for wastewater reuse. This field study therefore provides new evidence onthe fate of bacteriophages and a first indication of their potential use for performance evaluation in TF and aerated ConstructedWetlands. It also demonstrates that the combination
of TF with aerated constructed wetlands could be a novel and effective treatment scheme for new STWor for the upgrade of existing STW.
Original languageEnglish
Pages (from-to)1135-1145
Number of pages10
JournalScience of the Total Environment
Volume659
DOIs
Publication statusPublished - 29 Dec 2018

Fingerprint

bacteriophage
constructed wetland
bacterium
sewage treatment
wetland
fecal coliform
RNA
pathogen
trickling filter
sedimentation
effluent
wastewater
indicator
removal

Keywords

  • Aerated constructed wetland
  • Trickling filter
  • Bacteriophages
  • Wastewater reuse
  • Sewage Treatment Works
  • Indicator bacteria

Cite this

Stefanakis, Alexandros ; Bardiau, Marjorie ; Gomes Da Silva, Diogo ; Couceiro, F. ; Williams, J.B. ; Taylor, Huw. / Presence of bacteria and bacteriophages in full-scale trickling filters and an aerated constructed wetland. In: Science of the Total Environment. 2018 ; Vol. 659. pp. 1135-1145.
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abstract = "Aerated Constructed Wetlands are a state-of-the-art design that provides a different physical and chemical environment (compared to traditional passive wetland designs) for the wastewater treatment processes and, thus, mayhave different pathogen removal characteristics. In order to establish the fate of bacterial and viral indicators, a field studywas carried out at a Sewage Treatment Works (STW) in the UK (serving 20,000 pe). The STWconsists of primaryand secondary sedimentation tanks and tricklingfilters (TF) as the biological stage.Alarge (1,160m2) pilot aerated Vertical Flow Constructed Wetland (AVFCW) was constructed at the STW as tertiary stage receiving ¼ of the total flowrate, i.e., 1250m3/day. Effluent quality of the AVFCW complied with national and international standards for environmental discharge and reuse. For the first time, two sets of bacterial (Faecal coliforms, E.coli and intestinal enterococci)and viral indicators (Somatic coliphages, F-RNA specific bacteriophages and human-specific B. fragilis GB124 phages) were simultaneously investigated in an AVFCW and TF. High elimination rates were detected (up to 3.7 and 2.2 log reduction for bacteria indicators and phages, respectively) and strong correlations between the two sets were found. The superior efficiency of the aerated ConstructedWetlands inmicrobiological contamination removal compared to passive wetland systems was established for the first time, whichmay have implications for process selection for wastewater reuse. This field study therefore provides new evidence onthe fate of bacteriophages and a first indication of their potential use for performance evaluation in TF and aerated ConstructedWetlands. It also demonstrates that the combinationof TF with aerated constructed wetlands could be a novel and effective treatment scheme for new STWor for the upgrade of existing STW.",
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Presence of bacteria and bacteriophages in full-scale trickling filters and an aerated constructed wetland. / Stefanakis, Alexandros; Bardiau, Marjorie; Gomes Da Silva, Diogo; Couceiro, F.; Williams, J.B.; Taylor, Huw.

In: Science of the Total Environment, Vol. 659, 29.12.2018, p. 1135-1145.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Presence of bacteria and bacteriophages in full-scale trickling filters and an aerated constructed wetland

AU - Stefanakis, Alexandros

AU - Bardiau, Marjorie

AU - Gomes Da Silva, Diogo

AU - Couceiro, F.

AU - Williams, J.B.

AU - Taylor, Huw

PY - 2018/12/29

Y1 - 2018/12/29

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AB - Aerated Constructed Wetlands are a state-of-the-art design that provides a different physical and chemical environment (compared to traditional passive wetland designs) for the wastewater treatment processes and, thus, mayhave different pathogen removal characteristics. In order to establish the fate of bacterial and viral indicators, a field studywas carried out at a Sewage Treatment Works (STW) in the UK (serving 20,000 pe). The STWconsists of primaryand secondary sedimentation tanks and tricklingfilters (TF) as the biological stage.Alarge (1,160m2) pilot aerated Vertical Flow Constructed Wetland (AVFCW) was constructed at the STW as tertiary stage receiving ¼ of the total flowrate, i.e., 1250m3/day. Effluent quality of the AVFCW complied with national and international standards for environmental discharge and reuse. For the first time, two sets of bacterial (Faecal coliforms, E.coli and intestinal enterococci)and viral indicators (Somatic coliphages, F-RNA specific bacteriophages and human-specific B. fragilis GB124 phages) were simultaneously investigated in an AVFCW and TF. High elimination rates were detected (up to 3.7 and 2.2 log reduction for bacteria indicators and phages, respectively) and strong correlations between the two sets were found. The superior efficiency of the aerated ConstructedWetlands inmicrobiological contamination removal compared to passive wetland systems was established for the first time, whichmay have implications for process selection for wastewater reuse. This field study therefore provides new evidence onthe fate of bacteriophages and a first indication of their potential use for performance evaluation in TF and aerated ConstructedWetlands. It also demonstrates that the combinationof TF with aerated constructed wetlands could be a novel and effective treatment scheme for new STWor for the upgrade of existing STW.

KW - Aerated constructed wetland

KW - Trickling filter

KW - Bacteriophages

KW - Wastewater reuse

KW - Sewage Treatment Works

KW - Indicator bacteria

U2 - 10.1016/j.scitotenv.2018.12.415

DO - 10.1016/j.scitotenv.2018.12.415

M3 - Article

VL - 659

SP - 1135

EP - 1145

JO - Science of the Total Environment

JF - Science of the Total Environment

SN - 0048-9697

ER -