Preparation of liposomes containing small gold nanoparticles using electrostatic interactions

Gennaro Dichello, Ananth Pannala, T. Fukuda, T. Maekawa, Raymond Whitby, Sergey Mikhalovsky, M. Alavijeh, Dipak Sarker

Research output: Contribution to journalArticle

Abstract

The development of liposome-nanoparticle colloid systems offers a versatile approach towards the manufactureof multifunctional therapeutic platforms. A strategy to encapsulate small metallic nanoparticles (< 4 nm) withinmultilamellar vesicles, effected by exploiting electrostatic interactions was investigated. Two liposome-goldnanoparticle (lipo-GNP) systems were prepared by the reverse-phase evaporation method employing cationic oranionic surface functionalised particles in combination with oppositely charged lipid compositions withsubsequent post-formulation PEGylation. Structural characterisation using electron microscopy and elementalanalysis revealed a regular distribution of GNPs between adjacent lipid bilayers of intact liposomes. Nanoparticleencapsulation efficacy of the two lipo-GNP systems was revealed to be significantly different (p=0.03),evaluated by comparing the ratio of measured lipid to gold concentration (loading content) determined by acolorimetric assay and atomic emission spectroscopy, respectively. It was concluded that the developed syntheticstrategy is an effective approach for the preparation of liposome-nanoparticle colloids with potential to controlthe relative concentration of encapsulated particles to lipids by providing favourable electrostatic interactions.
Original languageEnglish
Pages (from-to)55-63
Number of pages9
JournalEuropean Journal of Pharmaceutical Sciences
Volume105
DOIs
Publication statusPublished - 3 May 2017

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  • Activities

    • 1 Research degree
    • 1 Invited talk

    Masters Degree by Research (France) - Mr Victor Jasny (University of Tours at Blois)

    Dipak Sarker (Supervisor)

    1 Mar 20201 Aug 2020

    Activity: External examination and supervisionResearch degree

    Nanosystems for in-vivo self-assembling medical devices.

    Dipak Sarker (Presenter)

    14 Nov 201415 Nov 2014

    Activity: External talk or presentationInvited talk

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