Prenatal alcohol exposure reduces 5-HT concentration in mouse intestinal muscle and mucosa

Katarzyna A. Dylag, Sara Fidalgo, Paul Gard, Bhavik Patel

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The influence of prenatal alcohol exposure on theserotoninergic system in the brain has been well studied, however its influence on the serotoninergic system in the gastrointestinal system remains unknown. The objective of the study was to use a mouse model of prenatal alcohol exposure to investigate the effects on serotonin and its metabolites and precursors in colonic tissue. This study used treatment of mouse breeding harems with 5% ethanol with saccharin via drinking water throughout pregnancy and compared the results with a saccharin control group. Tryptophan, serotonin (5-HT) and 5- hydroxyindoleacetic acid (5-HIAA) concentrations were measured in the longitudinal muscle myenteric plexus (LMMP) and mucosa of intestinal tissue by high- performance liquid chromatography (HPLC). Decreased 5-HT concentrations in mucosa and LMMP (females only) were observed in prenatally exposed mice compared to controls. Increases in mucosal and LMMP tryptophan concentration were only observed in prenatally exposed female mice. In conclusion, prenatal alcohol exposure causes a decrease in conversion of tryptophan to 5-HT in both muscle and mucosa although the effect is more pronounced in females. The observed sex difference may be related tochanges associated with the estrous cycle.
Original languageEnglish
JournalEnvironmental Toxicology and Pharmacology
DOIs
Publication statusPublished - 22 May 2018

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Intestinal Mucosa
Serotonin
Myenteric Plexus
Alcohols
Muscles
Tryptophan
Saccharin
Mucous Membrane
Hydroxyindoleacetic Acid
Estrous Cycle
Sex Characteristics
Drinking Water
Breeding
Ethanol
High Pressure Liquid Chromatography
Pregnancy
Control Groups
Brain
Therapeutics

Bibliographical note

© 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/

Keywords

  • 5-HT, serotonin
  • 5HIAA, 5-hydroxyindoleacetic acid
  • HPLC, high-performance liquid chromatograph
  • LMMP, longitudinal muscle myenteric plexus

Cite this

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title = "Prenatal alcohol exposure reduces 5-HT concentration in mouse intestinal muscle and mucosa",
abstract = "The influence of prenatal alcohol exposure on theserotoninergic system in the brain has been well studied, however its influence on the serotoninergic system in the gastrointestinal system remains unknown. The objective of the study was to use a mouse model of prenatal alcohol exposure to investigate the effects on serotonin and its metabolites and precursors in colonic tissue. This study used treatment of mouse breeding harems with 5{\%} ethanol with saccharin via drinking water throughout pregnancy and compared the results with a saccharin control group. Tryptophan, serotonin (5-HT) and 5- hydroxyindoleacetic acid (5-HIAA) concentrations were measured in the longitudinal muscle myenteric plexus (LMMP) and mucosa of intestinal tissue by high- performance liquid chromatography (HPLC). Decreased 5-HT concentrations in mucosa and LMMP (females only) were observed in prenatally exposed mice compared to controls. Increases in mucosal and LMMP tryptophan concentration were only observed in prenatally exposed female mice. In conclusion, prenatal alcohol exposure causes a decrease in conversion of tryptophan to 5-HT in both muscle and mucosa although the effect is more pronounced in females. The observed sex difference may be related tochanges associated with the estrous cycle.",
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Prenatal alcohol exposure reduces 5-HT concentration in mouse intestinal muscle and mucosa. / Dylag, Katarzyna A.; Fidalgo, Sara; Gard, Paul; Patel, Bhavik.

In: Environmental Toxicology and Pharmacology, 22.05.2018.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Prenatal alcohol exposure reduces 5-HT concentration in mouse intestinal muscle and mucosa

AU - Dylag, Katarzyna A.

AU - Fidalgo, Sara

AU - Gard, Paul

AU - Patel, Bhavik

N1 - © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/

PY - 2018/5/22

Y1 - 2018/5/22

N2 - The influence of prenatal alcohol exposure on theserotoninergic system in the brain has been well studied, however its influence on the serotoninergic system in the gastrointestinal system remains unknown. The objective of the study was to use a mouse model of prenatal alcohol exposure to investigate the effects on serotonin and its metabolites and precursors in colonic tissue. This study used treatment of mouse breeding harems with 5% ethanol with saccharin via drinking water throughout pregnancy and compared the results with a saccharin control group. Tryptophan, serotonin (5-HT) and 5- hydroxyindoleacetic acid (5-HIAA) concentrations were measured in the longitudinal muscle myenteric plexus (LMMP) and mucosa of intestinal tissue by high- performance liquid chromatography (HPLC). Decreased 5-HT concentrations in mucosa and LMMP (females only) were observed in prenatally exposed mice compared to controls. Increases in mucosal and LMMP tryptophan concentration were only observed in prenatally exposed female mice. In conclusion, prenatal alcohol exposure causes a decrease in conversion of tryptophan to 5-HT in both muscle and mucosa although the effect is more pronounced in females. The observed sex difference may be related tochanges associated with the estrous cycle.

AB - The influence of prenatal alcohol exposure on theserotoninergic system in the brain has been well studied, however its influence on the serotoninergic system in the gastrointestinal system remains unknown. The objective of the study was to use a mouse model of prenatal alcohol exposure to investigate the effects on serotonin and its metabolites and precursors in colonic tissue. This study used treatment of mouse breeding harems with 5% ethanol with saccharin via drinking water throughout pregnancy and compared the results with a saccharin control group. Tryptophan, serotonin (5-HT) and 5- hydroxyindoleacetic acid (5-HIAA) concentrations were measured in the longitudinal muscle myenteric plexus (LMMP) and mucosa of intestinal tissue by high- performance liquid chromatography (HPLC). Decreased 5-HT concentrations in mucosa and LMMP (females only) were observed in prenatally exposed mice compared to controls. Increases in mucosal and LMMP tryptophan concentration were only observed in prenatally exposed female mice. In conclusion, prenatal alcohol exposure causes a decrease in conversion of tryptophan to 5-HT in both muscle and mucosa although the effect is more pronounced in females. The observed sex difference may be related tochanges associated with the estrous cycle.

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KW - 5HIAA, 5-hydroxyindoleacetic acid

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