Plume characteristics and dynamics of UV and IR laser-desorbed oligonucleotides

Tony L. Merrigan, David J. Timson, C. Adam Hunniford, Martin Catney, Robert W. McCullough

Research output: Contribution to journalArticlepeer-review

Abstract

Laser desorption of dye-tagged oligonucleotides was studied using laser-induced fluorescence imaging. Desorption with ultra violet (UV) and infra-red (IR) lasers resulted in forward directed plumes of molecules. In the case of UV desorption, the initial shot desorbed approximately seven-fold more material than subsequent shots. In contrast, the initial shot in IR desorption resulted in the ejection of less material compared to subsequent shots and these plumes had a component directed along the path of the laser. Thermal equilibrium of the molecules in the plume was achieved after approximately 25 μs with a spread in molecular temperature which was described by a modified Maxwell-Boltzmann equation.

Original languageEnglish
Pages (from-to)1081-1090
Number of pages10
JournalInternational Journal of Biological Macromolecules
Volume50
Issue number4
DOIs
Publication statusPublished - 1 May 2012

Keywords

  • First shot effect
  • Laser desorption
  • Oligonucleotides
  • Plume dynamics

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