Planar lipid bilayers in recombinant ion channel research

Jacqueline Maher; Marcus Allen

doi:10.1016/j.ymeth.2018.03.003

Planar lipid bilayers in recombinant ion channel research

Jacqueline Maher, Marcus Allen

Research output: Contribution to journal › Article › peer-review

Abstract

There are a number of methods of investigating the function of recombinant proteins such as ion channels. However, after channel purification there are few methods to guarantee that the protein still functions. For ion channels, reconstituting back into planar lipid bilayers and demonstrating preserved function is a convenient and trusted method. It is cell free and even inaccessible, intracellular ion channels can be studied. We have used this method to study the function of recombinant channels of known subunit composition and have found it convenient for investigating the mode of action of ion channel modulators.

Original language	English
Pages (from-to)	206-212
Journal	Methods
Volume	147
DOIs	https://doi.org/10.1016/j.ymeth.2018.03.003
Publication status	Published - 9 Mar 2018

Bibliographical note

Keywords

Planar lipid bilayer
BK channel
Single channel gating
Lipids
Electrophysiology
Pharmacology

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1-s2.0-S1046202317304498-main.pdfAccepted author manuscript, 610 KBLicence: CC BY-NC-ND

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AU - Allen, Marcus

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AB - There are a number of methods of investigating the function of recombinant proteins such as ion channels. However, after channel purification there are few methods to guarantee that the protein still functions. For ion channels, reconstituting back into planar lipid bilayers and demonstrating preserved function is a convenient and trusted method. It is cell free and even inaccessible, intracellular ion channels can be studied. We have used this method to study the function of recombinant channels of known subunit composition and have found it convenient for investigating the mode of action of ion channel modulators.

KW - Planar lipid bilayer

KW - BK channel

KW - Single channel gating

KW - Lipids

KW - Electrophysiology

KW - Pharmacology

U2 - 10.1016/j.ymeth.2018.03.003

DO - 10.1016/j.ymeth.2018.03.003

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JO - Methods

JF - Methods

ER -

Planar lipid bilayers in recombinant ion channel research

Abstract

Bibliographical note

Keywords

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