Planar lipid bilayers in recombinant ion channel research

Jacqueline Maher, Marcus Allen

Research output: Contribution to journalArticle

Abstract

There are a number of methods of investigating the function of recombinant proteins such as ion channels. However, after channel purification there are few methods to guarantee that the protein still functions. For ion channels, reconstituting back into planar lipid bilayers and demonstrating preserved function is a convenient and trusted method. It is cell free and even inaccessible, intracellular ion channels can be studied. We have used this method to study the function of recombinant channels of known subunit composition and have found it convenient for investigating the mode of action of ion channel modulators.
Original languageEnglish
Pages (from-to)206-212
JournalMethods
Volume147
DOIs
Publication statusPublished - 9 Mar 2018

Bibliographical note

© 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/

Keywords

  • Planar lipid bilayer
  • BK channel
  • Single channel gating
  • Lipids
  • Electrophysiology
  • Pharmacology

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