One-step formation of three-dimensional macroporous bacterial sponges as a novel approach for the preparation of bioreactors for bioremediation and green treatment of water

Areej K. Al-Jwaid, Dmitriy Berillo, Irina Savina, Cundy Andrew, Jonathan Caplin

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Immobilisation of bacteria on or into a polymer support is a common method for the utilisation of bacteria
as biocatalysts for many biotechnological, medical and environmental applications. The main challenge in
this approach is the time taken for the formation of stable biofilms, and the typically low percentage of
bacterial cells present on or in the polymer matrix. In this work we propose a novel method for
producing a porous bacteria based structure with the properties of a sponge (bacterial sponge) that we
then use as a bioreactor for water treatment. Cryogelation has been used as a tool to create
macroporous (i.e. with pores in the range 10–100 mm), highly permeable systems with low diffusion
constraints and high bacterial content (more than 98% to total material content). A novel crosslinking
system was used to form stable bacterial sponges with a high percentage of live bacteria organized in
a 3D porous structure. The bacterial sponge was produced in a one step process and can be made from
one or several bacterial strains (in this case, two bacterial strains Pseudomonas mendocina and
Rhodoccocus koreensis (and a mixture of both) were used). Reduction of the total polymer content to
2% makes the system more sustainable and environmentally friendly under disposal as it can be simply composted. The bacterial sponges have good mechanical stability and cell viability, which enables repeated use of the materials for phenol degradation for up to five weeks. The material can be stored and transported in cryogenic conditions (80 C) for prolonged periods of time, retaining its bioremediation activity following 4–6 weeks of frozen storage. The proposed method of producing bioreactors with a high number of live immobilised bacteria, low polymer content and controlled 3D structure is a promising tool for developing novel materials based on active bacterial cells for various environmental, biotechnological, biological and medical applications.
Original languageEnglish
Pages (from-to)30813-30824
Number of pages12
JournalRSC Advances
Volume8
Issue number54
DOIs
Publication statusPublished - 3 Sep 2018

Fingerprint

Environmental Biodegradation
Bioremediation
Water Purification
Porifera
Bioreactors
sponge
bioremediation
bioreactor
Bacteria
Polymers
polymer
Water
bacterium
Pseudomonas mendocina
water
Biocatalysts
Mechanical stability
Biofilms
Medical applications
Phenol

Bibliographical note

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence

Keywords

  • macroporous
  • bioreactor
  • cryogel
  • bacterial sponge
  • bioremediation

Cite this

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title = "One-step formation of three-dimensional macroporous bacterial sponges as a novel approach for the preparation of bioreactors for bioremediation and green treatment of water",
abstract = "Immobilisation of bacteria on or into a polymer support is a common method for the utilisation of bacteriaas biocatalysts for many biotechnological, medical and environmental applications. The main challenge inthis approach is the time taken for the formation of stable biofilms, and the typically low percentage ofbacterial cells present on or in the polymer matrix. In this work we propose a novel method forproducing a porous bacteria based structure with the properties of a sponge (bacterial sponge) that wethen use as a bioreactor for water treatment. Cryogelation has been used as a tool to createmacroporous (i.e. with pores in the range 10–100 mm), highly permeable systems with low diffusionconstraints and high bacterial content (more than 98{\%} to total material content). A novel crosslinkingsystem was used to form stable bacterial sponges with a high percentage of live bacteria organized ina 3D porous structure. The bacterial sponge was produced in a one step process and can be made fromone or several bacterial strains (in this case, two bacterial strains Pseudomonas mendocina andRhodoccocus koreensis (and a mixture of both) were used). Reduction of the total polymer content to2{\%} makes the system more sustainable and environmentally friendly under disposal as it can be simply composted. The bacterial sponges have good mechanical stability and cell viability, which enables repeated use of the materials for phenol degradation for up to five weeks. The material can be stored and transported in cryogenic conditions (80 C) for prolonged periods of time, retaining its bioremediation activity following 4–6 weeks of frozen storage. The proposed method of producing bioreactors with a high number of live immobilised bacteria, low polymer content and controlled 3D structure is a promising tool for developing novel materials based on active bacterial cells for various environmental, biotechnological, biological and medical applications.",
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One-step formation of three-dimensional macroporous bacterial sponges as a novel approach for the preparation of bioreactors for bioremediation and green treatment of water. / K. Al-Jwaid, Areej; Berillo, Dmitriy; Savina, Irina; Andrew, Cundy; Caplin, Jonathan.

In: RSC Advances, Vol. 8, No. 54, 03.09.2018, p. 30813-30824.

Research output: Contribution to journalArticleResearchpeer-review

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AU - K. Al-Jwaid, Areej

AU - Berillo, Dmitriy

AU - Savina, Irina

AU - Andrew, Cundy

AU - Caplin, Jonathan

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KW - bioremediation

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