Novel cationic lipopolyplexes as gene therapy vectors

Atefeh Mohammadi, Katharina Welser, Laila Kudsiova, Frederick Campbell, Natalie Dawson, Jayne M. Lawrence, Alethea B. Tabor, Helen C. Hailes

Research output: Contribution to conferenceAbstract

Abstract

A major obstacle in the development of gene therapy is delivery of therapeutic genes to the desired cell/tissue. The objective of our study is to use a non-viral ternary system (lipopolyplexes) to encapsulate and deliver therapeutic DNA. Our lipopolyplexes comprise a glycerol-based cytofectin, a targeting peptide and plasmid DNA. Novel derivatives of the cationic lipids DOTMA and DOTAP have been synthesized and tested in a breast cancer cell line. A range of branched cationic peptides varying in number of residues, composition and linker to a targeting head group were also designed and prepared. The bio-physical studies demonstrated that all LPD complexes were positively charged, small (60-80 nm) and were shown to effectively condense DNA. Gel assays showed which peptides were able to protect DNA more effectively and gave high transfection efficiency. Further studies are underway investigating these systems in siRNA delivery.
Original languageEnglish
Pages57-57
Number of pages1
Publication statusPublished - 28 Aug 2011
Event242nd National Meeting of the American-Chemical-Society (ACS) - Denver, CO, 2011
Duration: 28 Aug 2011 → …

Conference

Conference242nd National Meeting of the American-Chemical-Society (ACS)
Period28/08/11 → …

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