Novel cationic lipopolyplexes as gene therapy vectors

Atefeh Mohammadi, Katharina Welser, Laila Kudsiova, Frederick Campbell, Natalie Dawson, Jayne M. Lawrence, Alethea B. Tabor, Helen C. Hailes

Research output: Contribution to conferenceAbstract

Abstract

A major obstacle in the development of gene therapy is delivery of therapeutic genes to the desired cell/tissue. The objective of our study is to use a non-viral ternary system (lipopolyplexes) to encapsulate and deliver therapeutic DNA. Our lipopolyplexes comprise a glycerol-based cytofectin, a targeting peptide and plasmid DNA. Novel derivatives of the cationic lipids DOTMA and DOTAP have been synthesized and tested in a breast cancer cell line. A range of branched cationic peptides varying in number of residues, composition and linker to a targeting head group were also designed and prepared. The bio-physical studies demonstrated that all LPD complexes were positively charged, small (60-80 nm) and were shown to effectively condense DNA. Gel assays showed which peptides were able to protect DNA more effectively and gave high transfection efficiency. Further studies are underway investigating these systems in siRNA delivery.
Original languageEnglish
Pages57-57
Number of pages1
Publication statusPublished - 28 Aug 2011
Event242nd National Meeting of the American-Chemical-Society (ACS) - Denver, CO, 2011
Duration: 28 Aug 2011 → …

Conference

Conference242nd National Meeting of the American-Chemical-Society (ACS)
Period28/08/11 → …

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Genetic Therapy
DNA
Peptides
Glycerol
Small Interfering RNA
Transfection
Plasmids
Gels
Breast Neoplasms
Lipids
Cell Line
Therapeutics
Genes

Cite this

Mohammadi, A., Welser, K., Kudsiova, L., Campbell, F., Dawson, N., Lawrence, J. M., ... Hailes, H. C. (2011). Novel cationic lipopolyplexes as gene therapy vectors. 57-57. Abstract from 242nd National Meeting of the American-Chemical-Society (ACS), .
Mohammadi, Atefeh ; Welser, Katharina ; Kudsiova, Laila ; Campbell, Frederick ; Dawson, Natalie ; Lawrence, Jayne M. ; Tabor, Alethea B. ; Hailes, Helen C. / Novel cationic lipopolyplexes as gene therapy vectors. Abstract from 242nd National Meeting of the American-Chemical-Society (ACS), .1 p.
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author = "Atefeh Mohammadi and Katharina Welser and Laila Kudsiova and Frederick Campbell and Natalie Dawson and Lawrence, {Jayne M.} and Tabor, {Alethea B.} and Hailes, {Helen C.}",
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Mohammadi, A, Welser, K, Kudsiova, L, Campbell, F, Dawson, N, Lawrence, JM, Tabor, AB & Hailes, HC 2011, 'Novel cationic lipopolyplexes as gene therapy vectors' 242nd National Meeting of the American-Chemical-Society (ACS), 28/08/11, pp. 57-57.

Novel cationic lipopolyplexes as gene therapy vectors. / Mohammadi, Atefeh; Welser, Katharina; Kudsiova, Laila; Campbell, Frederick; Dawson, Natalie; Lawrence, Jayne M.; Tabor, Alethea B.; Hailes, Helen C.

2011. 57-57 Abstract from 242nd National Meeting of the American-Chemical-Society (ACS), .

Research output: Contribution to conferenceAbstract

TY - CONF

T1 - Novel cationic lipopolyplexes as gene therapy vectors

AU - Mohammadi, Atefeh

AU - Welser, Katharina

AU - Kudsiova, Laila

AU - Campbell, Frederick

AU - Dawson, Natalie

AU - Lawrence, Jayne M.

AU - Tabor, Alethea B.

AU - Hailes, Helen C.

PY - 2011/8/28

Y1 - 2011/8/28

N2 - A major obstacle in the development of gene therapy is delivery of therapeutic genes to the desired cell/tissue. The objective of our study is to use a non-viral ternary system (lipopolyplexes) to encapsulate and deliver therapeutic DNA. Our lipopolyplexes comprise a glycerol-based cytofectin, a targeting peptide and plasmid DNA. Novel derivatives of the cationic lipids DOTMA and DOTAP have been synthesized and tested in a breast cancer cell line. A range of branched cationic peptides varying in number of residues, composition and linker to a targeting head group were also designed and prepared. The bio-physical studies demonstrated that all LPD complexes were positively charged, small (60-80 nm) and were shown to effectively condense DNA. Gel assays showed which peptides were able to protect DNA more effectively and gave high transfection efficiency. Further studies are underway investigating these systems in siRNA delivery.

AB - A major obstacle in the development of gene therapy is delivery of therapeutic genes to the desired cell/tissue. The objective of our study is to use a non-viral ternary system (lipopolyplexes) to encapsulate and deliver therapeutic DNA. Our lipopolyplexes comprise a glycerol-based cytofectin, a targeting peptide and plasmid DNA. Novel derivatives of the cationic lipids DOTMA and DOTAP have been synthesized and tested in a breast cancer cell line. A range of branched cationic peptides varying in number of residues, composition and linker to a targeting head group were also designed and prepared. The bio-physical studies demonstrated that all LPD complexes were positively charged, small (60-80 nm) and were shown to effectively condense DNA. Gel assays showed which peptides were able to protect DNA more effectively and gave high transfection efficiency. Further studies are underway investigating these systems in siRNA delivery.

M3 - Abstract

SP - 57

EP - 57

ER -

Mohammadi A, Welser K, Kudsiova L, Campbell F, Dawson N, Lawrence JM et al. Novel cationic lipopolyplexes as gene therapy vectors. 2011. Abstract from 242nd National Meeting of the American-Chemical-Society (ACS), .