Non-viral, coated nanoparticles as vectors for gene therapy

Frederick Campbell, Katharina Welser, Atefeh Mohammadi, Laila Kudsiova, Jayne M. Lawrence, Alethea B. Tabor, Helen C. Hailes

Research output: Contribution to conferenceAbstract

Abstract

Successful non-viral gene therapy is driven by the molecular makeup and architecture of the gene vector. For optimal delivery, consideration must be given to payload packaging/release, vector distribution/stability, cell-specific targeting and the physical properties of the therapeutic. Our focus is the construction of 'coated' liposomes for the targeted delivery of siRNA and DNA. In this presentation, we show small nanoparticles containing DNA/siRNA can be successfully formulated and coated with polyethylene glycol and other modified polymers. We also demonstrate cell specific targeting on addition of target peptides to the liposome coat. Furthermore, we demonstrate the role of novel branched, linear and cleavable polycationic peptides in payload packaging and intracellular release within our liposomal system. The chemical synthesis, biophysical properties andin vitrotransfection efficiencies of these systems will be fully discussed in this presentation.
Original languageEnglish
Pages107-107
Number of pages1
Publication statusPublished - 28 Aug 2011
Event242nd National Meeting of the American-Chemical-Society (ACS) - Denver, CO, 2011
Duration: 28 Aug 2011 → …

Conference

Conference242nd National Meeting of the American-Chemical-Society (ACS)
Period28/08/11 → …

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    Campbell, F., Welser, K., Mohammadi, A., Kudsiova, L., Lawrence, J. M., Tabor, A. B., & Hailes, H. C. (2011). Non-viral, coated nanoparticles as vectors for gene therapy. 107-107. Abstract from 242nd National Meeting of the American-Chemical-Society (ACS), .