Non-viral, coated nanoparticles as vectors for gene therapy

Frederick Campbell, Katharina Welser, Atefeh Mohammadi, Laila Kudsiova, Jayne M. Lawrence, Alethea B. Tabor, Helen C. Hailes

Research output: Contribution to conferenceAbstract

Abstract

Successful non-viral gene therapy is driven by the molecular makeup and architecture of the gene vector. For optimal delivery, consideration must be given to payload packaging/release, vector distribution/stability, cell-specific targeting and the physical properties of the therapeutic. Our focus is the construction of 'coated' liposomes for the targeted delivery of siRNA and DNA. In this presentation, we show small nanoparticles containing DNA/siRNA can be successfully formulated and coated with polyethylene glycol and other modified polymers. We also demonstrate cell specific targeting on addition of target peptides to the liposome coat. Furthermore, we demonstrate the role of novel branched, linear and cleavable polycationic peptides in payload packaging and intracellular release within our liposomal system. The chemical synthesis, biophysical properties andin vitrotransfection efficiencies of these systems will be fully discussed in this presentation.
Original languageEnglish
Pages107-107
Number of pages1
Publication statusPublished - 28 Aug 2011
Event242nd National Meeting of the American-Chemical-Society (ACS) - Denver, CO, 2011
Duration: 28 Aug 2011 → …

Conference

Conference242nd National Meeting of the American-Chemical-Society (ACS)
Period28/08/11 → …

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Product Packaging
Liposomes
Genetic Therapy
Nanoparticles
Small Interfering RNA
Peptides
DNA
Polymers
Genes
Therapeutics

Cite this

Campbell, F., Welser, K., Mohammadi, A., Kudsiova, L., Lawrence, J. M., Tabor, A. B., & Hailes, H. C. (2011). Non-viral, coated nanoparticles as vectors for gene therapy. 107-107. Abstract from 242nd National Meeting of the American-Chemical-Society (ACS), .
Campbell, Frederick ; Welser, Katharina ; Mohammadi, Atefeh ; Kudsiova, Laila ; Lawrence, Jayne M. ; Tabor, Alethea B. ; Hailes, Helen C. / Non-viral, coated nanoparticles as vectors for gene therapy. Abstract from 242nd National Meeting of the American-Chemical-Society (ACS), .1 p.
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author = "Frederick Campbell and Katharina Welser and Atefeh Mohammadi and Laila Kudsiova and Lawrence, {Jayne M.} and Tabor, {Alethea B.} and Hailes, {Helen C.}",
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Campbell, F, Welser, K, Mohammadi, A, Kudsiova, L, Lawrence, JM, Tabor, AB & Hailes, HC 2011, 'Non-viral, coated nanoparticles as vectors for gene therapy' 242nd National Meeting of the American-Chemical-Society (ACS), 28/08/11, pp. 107-107.

Non-viral, coated nanoparticles as vectors for gene therapy. / Campbell, Frederick; Welser, Katharina; Mohammadi, Atefeh; Kudsiova, Laila; Lawrence, Jayne M.; Tabor, Alethea B.; Hailes, Helen C.

2011. 107-107 Abstract from 242nd National Meeting of the American-Chemical-Society (ACS), .

Research output: Contribution to conferenceAbstract

TY - CONF

T1 - Non-viral, coated nanoparticles as vectors for gene therapy

AU - Campbell, Frederick

AU - Welser, Katharina

AU - Mohammadi, Atefeh

AU - Kudsiova, Laila

AU - Lawrence, Jayne M.

AU - Tabor, Alethea B.

AU - Hailes, Helen C.

PY - 2011/8/28

Y1 - 2011/8/28

N2 - Successful non-viral gene therapy is driven by the molecular makeup and architecture of the gene vector. For optimal delivery, consideration must be given to payload packaging/release, vector distribution/stability, cell-specific targeting and the physical properties of the therapeutic. Our focus is the construction of 'coated' liposomes for the targeted delivery of siRNA and DNA. In this presentation, we show small nanoparticles containing DNA/siRNA can be successfully formulated and coated with polyethylene glycol and other modified polymers. We also demonstrate cell specific targeting on addition of target peptides to the liposome coat. Furthermore, we demonstrate the role of novel branched, linear and cleavable polycationic peptides in payload packaging and intracellular release within our liposomal system. The chemical synthesis, biophysical properties andin vitrotransfection efficiencies of these systems will be fully discussed in this presentation.

AB - Successful non-viral gene therapy is driven by the molecular makeup and architecture of the gene vector. For optimal delivery, consideration must be given to payload packaging/release, vector distribution/stability, cell-specific targeting and the physical properties of the therapeutic. Our focus is the construction of 'coated' liposomes for the targeted delivery of siRNA and DNA. In this presentation, we show small nanoparticles containing DNA/siRNA can be successfully formulated and coated with polyethylene glycol and other modified polymers. We also demonstrate cell specific targeting on addition of target peptides to the liposome coat. Furthermore, we demonstrate the role of novel branched, linear and cleavable polycationic peptides in payload packaging and intracellular release within our liposomal system. The chemical synthesis, biophysical properties andin vitrotransfection efficiencies of these systems will be fully discussed in this presentation.

M3 - Abstract

SP - 107

EP - 107

ER -

Campbell F, Welser K, Mohammadi A, Kudsiova L, Lawrence JM, Tabor AB et al. Non-viral, coated nanoparticles as vectors for gene therapy. 2011. Abstract from 242nd National Meeting of the American-Chemical-Society (ACS), .