Successful non-viral gene therapy is driven by the molecular makeup and architecture of the gene vector. For optimal delivery, consideration must be given to payload packaging/release, vector distribution/stability, cell-specific targeting and the physical properties of the therapeutic. Our focus is the construction of 'coated' liposomes for the targeted delivery of siRNA and DNA. In this presentation, we show small nanoparticles containing DNA/siRNA can be successfully formulated and coated with polyethylene glycol and other modified polymers. We also demonstrate cell specific targeting on addition of target peptides to the liposome coat. Furthermore, we demonstrate the role of novel branched, linear and cleavable polycationic peptides in payload packaging and intracellular release within our liposomal system. The chemical synthesis, biophysical properties andin vitrotransfection efficiencies of these systems will be fully discussed in this presentation.
|Number of pages||1|
|Publication status||Published - 28 Aug 2011|
|Event||242nd National Meeting of the American-Chemical-Society (ACS) - Denver, CO, 2011|
Duration: 28 Aug 2011 → …
|Conference||242nd National Meeting of the American-Chemical-Society (ACS)|
|Period||28/08/11 → …|
Campbell, F., Welser, K., Mohammadi, A., Kudsiova, L., Lawrence, J. M., Tabor, A. B., & Hailes, H. C. (2011). Non-viral, coated nanoparticles as vectors for gene therapy. 107-107. Abstract from 242nd National Meeting of the American-Chemical-Society (ACS), .