Neurite outgrowths of neurons with neurotrophin-coated carbon nanotubes

K. Matsumoto; C. Sato; Y. Naka; A. Kitazawa; Raymond Whitby; N. Shimizu

doi:10.1263/jbb.103.216

Neurite outgrowths of neurons with neurotrophin-coated carbon nanotubes

K. Matsumoto, C. Sato, Y. Naka, A. Kitazawa, Raymond Whitby, N. Shimizu

University of Brighton

Research output: Contribution to journal › Article › peer-review

Abstract

Multiwalled carbon nanotubes (CNTs) coated with neurotrophin were used to regulate the differentiation and survival of neurons. Neurotrophin (nerve growth factor [NGF] or brain-derived neurotrophic factor [BDNF]) was covalently bound to CNTs modified by amino groups using a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) reagent. The CNTs coated with NGF or BDNF promoted the neurite outgrowths of neurons in the same manner as soluble NGF and soluble BDNF. By enzyme-linked immunosorbent assay (ELISA), we demonstrated that neurotrophin-coated CNTs carry neurotrophin. These results suggest that neurotrophin-coated CNTs have biological activity and stimulate the neurite outgrowths of neurons.

Original language	English
Pages (from-to)	216-220
Number of pages	5
Journal	Journal of Bioscience and Bioengineering
Volume	103
DOIs	https://doi.org/10.1263/jbb.103.216
Publication status	Published - Jan 2007

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title = "Neurite outgrowths of neurons with neurotrophin-coated carbon nanotubes",

abstract = "Multiwalled carbon nanotubes (CNTs) coated with neurotrophin were used to regulate the differentiation and survival of neurons. Neurotrophin (nerve growth factor [NGF] or brain-derived neurotrophic factor [BDNF]) was covalently bound to CNTs modified by amino groups using a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) reagent. The CNTs coated with NGF or BDNF promoted the neurite outgrowths of neurons in the same manner as soluble NGF and soluble BDNF. By enzyme-linked immunosorbent assay (ELISA), we demonstrated that neurotrophin-coated CNTs carry neurotrophin. These results suggest that neurotrophin-coated CNTs have biological activity and stimulate the neurite outgrowths of neurons.",

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AU - Matsumoto, K.

AU - Sato, C.

AU - Naka, Y.

AU - Kitazawa, A.

AU - Whitby, Raymond

AU - Shimizu, N.

PY - 2007/1

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N2 - Multiwalled carbon nanotubes (CNTs) coated with neurotrophin were used to regulate the differentiation and survival of neurons. Neurotrophin (nerve growth factor [NGF] or brain-derived neurotrophic factor [BDNF]) was covalently bound to CNTs modified by amino groups using a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) reagent. The CNTs coated with NGF or BDNF promoted the neurite outgrowths of neurons in the same manner as soluble NGF and soluble BDNF. By enzyme-linked immunosorbent assay (ELISA), we demonstrated that neurotrophin-coated CNTs carry neurotrophin. These results suggest that neurotrophin-coated CNTs have biological activity and stimulate the neurite outgrowths of neurons.

AB - Multiwalled carbon nanotubes (CNTs) coated with neurotrophin were used to regulate the differentiation and survival of neurons. Neurotrophin (nerve growth factor [NGF] or brain-derived neurotrophic factor [BDNF]) was covalently bound to CNTs modified by amino groups using a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) reagent. The CNTs coated with NGF or BDNF promoted the neurite outgrowths of neurons in the same manner as soluble NGF and soluble BDNF. By enzyme-linked immunosorbent assay (ELISA), we demonstrated that neurotrophin-coated CNTs carry neurotrophin. These results suggest that neurotrophin-coated CNTs have biological activity and stimulate the neurite outgrowths of neurons.

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VL - 103

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JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

ER -

Neurite outgrowths of neurons with neurotrophin-coated carbon nanotubes

Abstract

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