Monitoring gut epithelium serotonin and melatonin overflow provides spatial mapping of inflammation

Fernando Perez; Nikki Kotecha; Brigitte Lavoie; Gary M. Mawe; Bhavik Anil Patel

doi:10.1002/cbic.202200334

Monitoring gut epithelium serotonin and melatonin overflow provides spatial mapping of inflammation

Fernando Perez, Nikki Kotecha, Brigitte Lavoie, Gary M. Mawe, Bhavik Anil Patel

Research output: Contribution to journal › Article › peer-review

Abstract

Electrochemical arrays were used to measure the overflow of serotonin (5-HT) and melatonin (MEL) from the entire colon of healthy mice and mice with chemical-induced inflammatory bowel disease (IBD), to understand the interplay between inflammation and colonic function. We show that 5-HT overflow is increased, whilst MEL levels are reduced, in inflamed tissues. The levels of MEL are increased at the interface between healthy and inflamed regions within the colon and may limit the spread of inflammation. Understanding the interplay between inflammation and mucosal epithelial signalling can provide key insight into colonic function and aid the development of effective therapeutic strategies to treat gastrointestinal diseases.

Original language	English
Article number	e202200334
Pages (from-to)	e202200334
Number of pages	7
Journal	ChemBioChem
Volume	24
Issue number	2
DOIs	https://doi.org/10.1002/cbic.202200334
Publication status	Published - 17 Nov 2022

Bibliographical note

Funding Information:
B.A.P. was funded by IMPRESSPlus EPSRC grant (EP/N027345/1) and knowledge exchange award for funding part of this study. This work was funded in part by NIH grants AT011203 and DK113800 to G.M.M.

Publisher Copyright:
© 2022 The Authors. ChemBioChem published by Wiley-VCH GmbH.

Keywords

Organic Chemistry
Molecular Biology
Molecular Medicine
Biochemistry
serotonin
inflammation
Research Articles
melatonin
Research Article
electrode arrays
EC cells

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title = "Monitoring gut epithelium serotonin and melatonin overflow provides spatial mapping of inflammation",

abstract = "Electrochemical arrays were used to measure the overflow of serotonin (5-HT) and melatonin (MEL) from the entire colon of healthy mice and mice with chemical-induced inflammatory bowel disease (IBD), to understand the interplay between inflammation and colonic function. We show that 5-HT overflow is increased, whilst MEL levels are reduced, in inflamed tissues. The levels of MEL are increased at the interface between healthy and inflamed regions within the colon and may limit the spread of inflammation. Understanding the interplay between inflammation and mucosal epithelial signalling can provide key insight into colonic function and aid the development of effective therapeutic strategies to treat gastrointestinal diseases.",

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AU - Lavoie, Brigitte

AU - Mawe, Gary M.

AU - Patel, Bhavik Anil

N1 - Funding Information: B.A.P. was funded by IMPRESSPlus EPSRC grant (EP/N027345/1) and knowledge exchange award for funding part of this study. This work was funded in part by NIH grants AT011203 and DK113800 to G.M.M. Publisher Copyright: © 2022 The Authors. ChemBioChem published by Wiley-VCH GmbH.

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N2 - Electrochemical arrays were used to measure the overflow of serotonin (5-HT) and melatonin (MEL) from the entire colon of healthy mice and mice with chemical-induced inflammatory bowel disease (IBD), to understand the interplay between inflammation and colonic function. We show that 5-HT overflow is increased, whilst MEL levels are reduced, in inflamed tissues. The levels of MEL are increased at the interface between healthy and inflamed regions within the colon and may limit the spread of inflammation. Understanding the interplay between inflammation and mucosal epithelial signalling can provide key insight into colonic function and aid the development of effective therapeutic strategies to treat gastrointestinal diseases.

AB - Electrochemical arrays were used to measure the overflow of serotonin (5-HT) and melatonin (MEL) from the entire colon of healthy mice and mice with chemical-induced inflammatory bowel disease (IBD), to understand the interplay between inflammation and colonic function. We show that 5-HT overflow is increased, whilst MEL levels are reduced, in inflamed tissues. The levels of MEL are increased at the interface between healthy and inflamed regions within the colon and may limit the spread of inflammation. Understanding the interplay between inflammation and mucosal epithelial signalling can provide key insight into colonic function and aid the development of effective therapeutic strategies to treat gastrointestinal diseases.

KW - Organic Chemistry

KW - Molecular Biology

KW - Molecular Medicine

KW - Biochemistry

KW - serotonin

KW - inflammation

KW - Research Articles

KW - melatonin

KW - Research Article

KW - electrode arrays

KW - EC cells

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JO - ChemBioChem

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ER -

Monitoring gut epithelium serotonin and melatonin overflow provides spatial mapping of inflammation

Abstract

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Keywords

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