Electrochemical arrays were used to measure the overflow of serotonin (5-HT) and melatonin (MEL) from the entire colon of healthy mice and mice with chemical-induced inflammatory bowel disease (IBD), to understand the interplay between inflammation and colonic function. We show that 5-HT overflow is increased, whilst MEL levels are reduced, in inflamed tissues. The levels of MEL are increased at the interface between healthy and inflamed regions within the colon and may limit the spread of inflammation. Understanding the interplay between inflammation and mucosal epithelial signalling can provide key insight into colonic function and aid the development of effective therapeutic strategies to treat gastrointestinal diseases.
Bibliographical noteFunding Information:
B.A.P. was funded by IMPRESSPlus EPSRC grant (EP/N027345/1) and knowledge exchange award for funding part of this study. This work was funded in part by NIH grants AT011203 and DK113800 to G.M.M.
© 2022 The Authors. ChemBioChem published by Wiley-VCH GmbH.
- Organic Chemistry
- Molecular Biology
- Molecular Medicine
- Research Articles
- Research Article
- electrode arrays
- EC cells