Methods to quantify the biotin-binding capacity of streptavidin-coated polypropylene PCR plates

A.M. van der Valk; D.N. Howbrook; M.C. O'Shaughnessy; Dipak Sarker; S.C. Baker; A. Louwrier; Andrew Lloyd

doi:10.1023/A:1024951322955

Methods to quantify the biotin-binding capacity of streptavidin-coated polypropylene PCR plates

A.M. van der Valk, D.N. Howbrook, M.C. O'Shaughnessy, Dipak Sarker, S.C. Baker, A. Louwrier, Andrew Lloyd

University of Brighton

Research output: Contribution to journal › Article › peer-review

Abstract

The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respectively.

Original language	English
Pages (from-to)	1325-1328
Number of pages	4
Journal	Biotechnology Letters
Volume	25
Issue number	16
DOIs	https://doi.org/10.1023/A:1024951322955
Publication status	Published - Aug 2003

Keywords

alkaline phosphatase
biotin
fluorescein
polypropylene
streptavidin

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10.1023/A:1024951322955Licence: Unspecified

http://link.springer.com/article/10.1023%2FA%3A1024951322955Licence: Unspecified

1 Oral presentation

Applied Sciences slides - lunchtime seminar 2021
Sarker, D. (Presenter), Johnson, L. (Presenter) & Davidson, S. (Presenter)
5 May 2021
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Cite this

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title = "Methods to quantify the biotin-binding capacity of streptavidin-coated polypropylene PCR plates",

abstract = "The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respectively.",

keywords = "alkaline phosphatase, biotin, fluorescein, polypropylene, streptavidin",

author = "{van der Valk}, A.M. and D.N. Howbrook and M.C. O'Shaughnessy and Dipak Sarker and S.C. Baker and A. Louwrier and Andrew Lloyd",

year = "2003",

month = aug,

doi = "10.1023/A:1024951322955",

language = "English",

volume = "25",

pages = "1325--1328",

journal = "Biotechnology Letters",

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AU - van der Valk, A.M.

AU - Howbrook, D.N.

AU - O'Shaughnessy, M.C.

AU - Sarker, Dipak

AU - Baker, S.C.

AU - Louwrier, A.

AU - Lloyd, Andrew

PY - 2003/8

Y1 - 2003/8

N2 - The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respectively.

AB - The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respectively.

KW - alkaline phosphatase

KW - biotin

KW - fluorescein

KW - polypropylene

KW - streptavidin

U2 - 10.1023/A:1024951322955

DO - 10.1023/A:1024951322955

M3 - Article

SN - 1573-6776

VL - 25

SP - 1325

EP - 1328

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 16

ER -

Methods to quantify the biotin-binding capacity of streptavidin-coated polypropylene PCR plates

Abstract

Keywords

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