Methods to quantify the biotin-binding capacity of streptavidin-coated polypropylene PCR plates

A.M. van der Valk, D.N. Howbrook, M.C. O'Shaughnessy, Dipak Sarker, S.C. Baker, A. Louwrier, Andrew Lloyd

Research output: Contribution to journalArticle

Abstract

The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respectively.
Original languageEnglish
Pages (from-to)1325-1328
Number of pages4
JournalBiotechnology Letters
Volume25
Issue number16
DOIs
Publication statusPublished - Aug 2003

Keywords

  • alkaline phosphatase
  • biotin
  • fluorescein
  • polypropylene
  • streptavidin

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