The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respectively.
- alkaline phosphatase
van der Valk, A. M., Howbrook, D. N., O'Shaughnessy, M. C., Sarker, D., Baker, S. C., Louwrier, A., & Lloyd, A. (2003). Methods to quantify the biotin-binding capacity of streptavidin-coated polypropylene PCR plates. Biotechnology Letters, 25(16), 1325-1328. https://doi.org/10.1023/A:1024951322955