Methodological issues with the assessment of voluntary activation using transcranial magnetic stimulation in the knee extensors

Jeanne Dekerle, Paul Ansdell, Lisa Schäfer, Aaron Greenhouse-Tucknott, James Wrightson

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Purpose: The assessment of voluntary activation of the knee extensors using transcranial magnetic stimulation (VA TMS ) is routinely performed to assess the supraspinal function. Yet methodological scrutiny of the technique is scarce. The aim of the present study was to examine face validity and reliability of VA TMS and its two main determinants (superimposed twitch during a maximal voluntary contraction [SIT 100% ] and estimated resting twitch [ERT]). Methods: SIT 100%, ERT, and VA TMS were measured on ten healthy males (age 24 ± 5 years) before and following intermittent isometric fatiguing exercise on two separate occasions. Results: The findings indicated issues regarding the accuracy of ERT and suggested a three-point relationship should not be used to determine ERT. Reliabilities for VA TMS , SIT 100%, and ERT were acceptable pre- but much weaker post-exercise (especially for SIT 100% ). Despite statistically significant changes in main neuromuscular variables following the intermittent isometric fatiguing exercise (P < 0.05), when post-exercise reliability was considered, the exercise effect on VA TMS was smaller than the smallest detectable change in 18 of the 20 individual tests performed, and for the whole sample for one of two visits. Finally, maximal voluntary contraction was reduced significantly following the neuromuscular assessment (NMA) pre-exercise but recovered during the NMA post-exercise. Conclusion: This is the first study to demonstrate a lack of sensitivity of key neuromuscular measurements to exercise and to evidence both presence of neuromuscular fatigue following the NMA in itself, and recovery of the neuromuscular function during the NMA post-exercise. These results challenge the face validity of this routinely used protocol.

Original languageEnglish
Pages (from-to)991-1005
Number of pages15
JournalEuropean Journal of Applied Physiology
Volume119
Issue number4
DOIs
Publication statusPublished - 12 Feb 2019

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Transcranial Magnetic Stimulation
Reproducibility of Results
Knee
Exercise
Recovery of Function
Fatigue

Keywords

  • Neuromuscular fatigue
  • Central fatigue
  • Exercise
  • Isometric contraction
  • Isokinetic dynanometer

Cite this

Dekerle, Jeanne ; Ansdell, Paul ; Schäfer, Lisa ; Greenhouse-Tucknott, Aaron ; Wrightson, James. / Methodological issues with the assessment of voluntary activation using transcranial magnetic stimulation in the knee extensors. In: European Journal of Applied Physiology. 2019 ; Vol. 119, No. 4. pp. 991-1005.
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abstract = "Purpose: The assessment of voluntary activation of the knee extensors using transcranial magnetic stimulation (VA TMS ) is routinely performed to assess the supraspinal function. Yet methodological scrutiny of the technique is scarce. The aim of the present study was to examine face validity and reliability of VA TMS and its two main determinants (superimposed twitch during a maximal voluntary contraction [SIT 100{\%} ] and estimated resting twitch [ERT]). Methods: SIT 100{\%}, ERT, and VA TMS were measured on ten healthy males (age 24 ± 5 years) before and following intermittent isometric fatiguing exercise on two separate occasions. Results: The findings indicated issues regarding the accuracy of ERT and suggested a three-point relationship should not be used to determine ERT. Reliabilities for VA TMS , SIT 100{\%}, and ERT were acceptable pre- but much weaker post-exercise (especially for SIT 100{\%} ). Despite statistically significant changes in main neuromuscular variables following the intermittent isometric fatiguing exercise (P < 0.05), when post-exercise reliability was considered, the exercise effect on VA TMS was smaller than the smallest detectable change in 18 of the 20 individual tests performed, and for the whole sample for one of two visits. Finally, maximal voluntary contraction was reduced significantly following the neuromuscular assessment (NMA) pre-exercise but recovered during the NMA post-exercise. Conclusion: This is the first study to demonstrate a lack of sensitivity of key neuromuscular measurements to exercise and to evidence both presence of neuromuscular fatigue following the NMA in itself, and recovery of the neuromuscular function during the NMA post-exercise. These results challenge the face validity of this routinely used protocol.",
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Methodological issues with the assessment of voluntary activation using transcranial magnetic stimulation in the knee extensors. / Dekerle, Jeanne; Ansdell, Paul; Schäfer, Lisa; Greenhouse-Tucknott, Aaron; Wrightson, James.

In: European Journal of Applied Physiology, Vol. 119, No. 4, 12.02.2019, p. 991-1005.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Methodological issues with the assessment of voluntary activation using transcranial magnetic stimulation in the knee extensors

AU - Dekerle, Jeanne

AU - Ansdell, Paul

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AU - Greenhouse-Tucknott, Aaron

AU - Wrightson, James

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