TY - JOUR
T1 - Metabolomic profiling of recombinant erythropoietin (rHuEpo) in trained Caucasian athletes.
AU - Lima, Giscard
AU - Shurlock, Jonathan
AU - Wang, Guan
AU - Karanikolou, Antonia
AU - Sutehall, Shaun
AU - Pitsiladis, Yannis
AU - D’Alessandro, Angelo
PY - 2022/10/10
Y1 - 2022/10/10
N2 - Recombinant human erythropoietin (rHuEpo) is prohibited by the World Anti-Doping Agency but remains the drug of choice for many cheating athletes wishing to evade detection using current methods. The aim of this study was to identify a robust metabolomics signature of rHuEpo using an untargeted approach in blood (plasma and serum) and urine. Longitudinal study. University of Glasgow. Eighteen male participants regularly engaged in predominantly endurance-based activities, such as running, cycling, swimming, triathlon, and team sports, were recruited. Each participant received 50 IU·kg-1 body mass of rHuEpo subcutaneously every 2 days for 4 weeks. Samples were collected at baseline, during rHuEpo administration (over 4 weeks) and after rHuEpo administration (week 7-10). The samples were analyzed using hydrophilic interaction liquid chromatography mass spectrometry. Significant metabolic signatures of rHuEpo administration were identified in all biofluids tested in this study. Regarding metabolomics data, 488 plasma metabolites, 694 serum metabolites, and 1628 urinary metabolites were identified. Reproducible signatures of rHuEpo administration across all biofluids included alterations of pyrimidine metabolism (orotate and dihydroorotate) and acyl-carnitines (palmitoyl-carnitine and elaidic carnitine), metabolic pathways that are associated with erythropoiesis or erythrocyte membrane function, respectively. Preliminary metabolic signatures of rHuEpo administration were identified. Future studies will be required to validate these encouraging results in independent cohorts and with orthogonal techniques, such as integration of our data with signatures derived from other "omics" analyses of rHuEpo administration (eg, transcriptomics). [Abstract copyright: Copyright © 2022 Wolters Kluwer Health, Inc. All rights reserved.]
AB - Recombinant human erythropoietin (rHuEpo) is prohibited by the World Anti-Doping Agency but remains the drug of choice for many cheating athletes wishing to evade detection using current methods. The aim of this study was to identify a robust metabolomics signature of rHuEpo using an untargeted approach in blood (plasma and serum) and urine. Longitudinal study. University of Glasgow. Eighteen male participants regularly engaged in predominantly endurance-based activities, such as running, cycling, swimming, triathlon, and team sports, were recruited. Each participant received 50 IU·kg-1 body mass of rHuEpo subcutaneously every 2 days for 4 weeks. Samples were collected at baseline, during rHuEpo administration (over 4 weeks) and after rHuEpo administration (week 7-10). The samples were analyzed using hydrophilic interaction liquid chromatography mass spectrometry. Significant metabolic signatures of rHuEpo administration were identified in all biofluids tested in this study. Regarding metabolomics data, 488 plasma metabolites, 694 serum metabolites, and 1628 urinary metabolites were identified. Reproducible signatures of rHuEpo administration across all biofluids included alterations of pyrimidine metabolism (orotate and dihydroorotate) and acyl-carnitines (palmitoyl-carnitine and elaidic carnitine), metabolic pathways that are associated with erythropoiesis or erythrocyte membrane function, respectively. Preliminary metabolic signatures of rHuEpo administration were identified. Future studies will be required to validate these encouraging results in independent cohorts and with orthogonal techniques, such as integration of our data with signatures derived from other "omics" analyses of rHuEpo administration (eg, transcriptomics). [Abstract copyright: Copyright © 2022 Wolters Kluwer Health, Inc. All rights reserved.]
KW - Orthopedics and Sports Medicine
KW - Physical Therapy, Sports Therapy and Rehabilitation
U2 - 10.1097/JSM.0000000000001074
DO - 10.1097/JSM.0000000000001074
M3 - Article
C2 - 36731031
SN - 1536-3724
VL - 33
SP - e123-e134
JO - Clinical Journal of Sport Medicine
JF - Clinical Journal of Sport Medicine
IS - 5
ER -