Mechanosensing is critical for axon growth in the developing brain

David E. Kosler, Amelia J. Thompson, Sarah K. Foster, Asha Dwivedy, Eva K. Pillai, Graham Sheridan, Hanno Svoboda, Matheus Viana, Luciano da F. Costa, Jochen Guck, Christine E. Holt, Kristian Franze

Research output: Contribution to journalArticleResearchpeer-review

Abstract

During nervous system development, neurons extend axons along well-defined pathways. The current understanding of axon pathfinding is based mainly on chemical signaling. However, growing neurons interact not only chemically but also mechanically with their environment. Here we identify mechanical signals as important regulators of axon pathfinding. In vitro, substrate stiffness determined growth patterns of Xenopus retinal ganglion cell axons. In vivo atomic force microscopy revealed a noticeable pattern of stiffness gradients in the embryonic brain. Retinal ganglion cell axons grew toward softer tissue, which was reproduced in vitro in the absence of chemical gradients. To test the importance of mechanical signals for axon growth in vivo, we altered brain stiffness, blocked mechanotransduction pharmacologically and knocked down the mechanosensitive ion channel piezo1. All treatments resulted in aberrant axonal growth and pathfinding errors, suggesting that local tissue stiffness, read out by mechanosensitive ion channels, is critically involved in instructing neuronal growth in vivo.
Original languageEnglish
JournalNature Neuroscience
DOIs
Publication statusPublished - 19 Sep 2016

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Axons
Retinal Ganglion Cells
Brain
Growth
Ion Channels
Neurons
Atomic Force Microscopy
Xenopus
Nervous System
Axon Guidance
In Vitro Techniques

Keywords

  • mechanosensitivity
  • durotaxis
  • AFM
  • axon guidance
  • biomechanics
  • stretch-activated ion channels
  • brain stiffness
  • stiffness gradient

Cite this

Kosler, D. E., Thompson, A. J., Foster, S. K., Dwivedy, A., Pillai, E. K., Sheridan, G., ... Franze, K. (2016). Mechanosensing is critical for axon growth in the developing brain. Nature Neuroscience. https://doi.org/10.1038/nn.4394
Kosler, David E. ; Thompson, Amelia J. ; Foster, Sarah K. ; Dwivedy, Asha ; Pillai, Eva K. ; Sheridan, Graham ; Svoboda, Hanno ; Viana, Matheus ; da F. Costa, Luciano ; Guck, Jochen ; Holt, Christine E. ; Franze, Kristian. / Mechanosensing is critical for axon growth in the developing brain. In: Nature Neuroscience. 2016.
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Kosler, DE, Thompson, AJ, Foster, SK, Dwivedy, A, Pillai, EK, Sheridan, G, Svoboda, H, Viana, M, da F. Costa, L, Guck, J, Holt, CE & Franze, K 2016, 'Mechanosensing is critical for axon growth in the developing brain' Nature Neuroscience. https://doi.org/10.1038/nn.4394

Mechanosensing is critical for axon growth in the developing brain. / Kosler, David E.; Thompson, Amelia J.; Foster, Sarah K.; Dwivedy, Asha; Pillai, Eva K.; Sheridan, Graham; Svoboda, Hanno; Viana, Matheus; da F. Costa, Luciano; Guck, Jochen; Holt, Christine E.; Franze, Kristian.

In: Nature Neuroscience, 19.09.2016.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Kosler, David E.

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AU - Sheridan, Graham

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AU - Franze, Kristian

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AB - During nervous system development, neurons extend axons along well-defined pathways. The current understanding of axon pathfinding is based mainly on chemical signaling. However, growing neurons interact not only chemically but also mechanically with their environment. Here we identify mechanical signals as important regulators of axon pathfinding. In vitro, substrate stiffness determined growth patterns of Xenopus retinal ganglion cell axons. In vivo atomic force microscopy revealed a noticeable pattern of stiffness gradients in the embryonic brain. Retinal ganglion cell axons grew toward softer tissue, which was reproduced in vitro in the absence of chemical gradients. To test the importance of mechanical signals for axon growth in vivo, we altered brain stiffness, blocked mechanotransduction pharmacologically and knocked down the mechanosensitive ion channel piezo1. All treatments resulted in aberrant axonal growth and pathfinding errors, suggesting that local tissue stiffness, read out by mechanosensitive ion channels, is critically involved in instructing neuronal growth in vivo.

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